PMID: 44317Jan 1, 1979

Some characteristics of early appearing isomaltase in intestinal mucosa of suckling rat

Journal of Nutritional Science and Vitaminology
K YamadaN Hosoya


Characteristics of early appearing free isomaltase in the soluble fraction were investigated in rat intestinal mucosa. Soluble isomaltase and membrane-bound sucrase-isomaltase complex were prepared from 15-day-old rat intestine. Immunochemical properties, optimal pH and heat sensitivity of soluble isomaltase were compared with those of membrane-bound isomaltase. Optimal pH of free isomaltase in the soluble fraction was lower than that of membrane-bound isomaltase. Soluble and membrane-bound isomaltase showed different sensitivities for temperature. Furthermore, membrane-bound isomaltase in 15-day-old suckling rat intestine gave a single line with antiserum. However, soluble isomaltase gave no precipitin line. From these results, it could be concluded that soluble isomaltase is not derived from the isomaltase moiety of membrane-bound sucrase-isomaltase complex as a result of mechanical fragility and rather it would be lysosomal in origin.

Related Concepts

Animals, Suckling
Immunodiffusion Measurement
Hydrogen-Ion Concentration
Structure of Intestinal Gland
Intracellular Membranes
Multienzyme Complexes
Plasma Protein Binding Capacity

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