PMID: 149Nov 1, 1975

Some characteristics of two azoreductase systems in rat liver. Relevance to the activity of 2-[4'-di(2"-bromopropyl)-aminophenylazo]benzoic acid (CB10-252), a compound possessing latent cytotoxic activity

Chemico-biological Interactions
H Autrup, G P Warwick

Abstract

The system involved in the reduction of 2-[4'-di(2''-bromopropyl) aminophenylazolbenzoic acid (CB10-252), an agent designed for treating primary liver cell cancer, has been demonstrated to be localised mainly in the 108 000 X g supernatant fraction of rat liver homogenate. It is also present in other organs particularly in the spleen. DAB-azoreductase as shown previously is present almost entirely in the microsomal fraction and is found in high concentration only in liver. The pH maximum for CB10-252-azoreductase implying the importance of the 2'-carboxyl group in determining substrate specificity. The use of enzyme inhibitors and other additives showed that CB10-252 WAS NOT AXANTHINE OXIDASE OR DIHYDROFOLATE REDUCTASE. Its activity was not affected by carbon monoxide, phenobarbitone (PB), or 3-methylcholanthrene (MC) pretreatment. Enhancement of the activity by ferrous ions and FAD indicated that at least part of the reduction system could involve a flavoprotein with FAD as the prosthetic group. The activity of CB10-252-azoreductase and methylred-azoreductase was reduced by menadione (vitamin K3), cyanide and propylgallate. A diaphorase preparation from pig heart reduced both CB10-252 and methylred with both NADPH- and NADH-ge...Continue Reading

Citations

Dec 1, 1987·Bulletin of Environmental Contamination and Toxicology·E IdakaT Ogawa
Jan 1, 1988·Journal of Toxicology and Environmental Health·S NesnowA Richard
Aug 29, 1978·Biochemical and Biophysical Research Communications·M T HuangA Y Lu
Jun 1, 1987·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·H RazaJ R Chowdhury
Sep 1, 1992·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·W G LevineS Zbaida
Jan 1, 1991·Drug Metabolism Reviews·W G Levine
Jan 1, 1979·International Journal of Health Services : Planning, Administration, Evaluation·H WaitzkinJ Sharratt
Feb 19, 2008·International Journal of Toxicology
Apr 1, 1980·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·K W Renton

Related Concepts

Metazoa
Azo Compounds
Cecum
Cell Fractionation
Cytoplasmic Matrix
Enzyme Induction
Flavitan
Flavin Mononucleotide Disodium Salt
Hydrogen-Ion Concentration
Liver

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