PMID: 459Jun 1, 1975

Some effects of low pH on chloride exchange in human red blood cells

The Journal of General Physiology
R B GunnD C Tosteson


In order to test the range of pH values over which the titratable carried model for inorganic anion exchange is valid, chloride self-exchange across human red blood cells was examined between pH 4.75 and 5.7 at 0 decrees c. It was found that chloride self-exchange flux had a minimum near pH 5 and increased again with further increase in hydrogen ion activity. The Arrhenius activation energy for chloride exchange was greatly reduced at low pH values. The chloride flux at pH 5.1 did not show the saturation kinetics reported at higher pH values but was proportional to the value of the chloride concentration squared. In addition, the extent of inhibition of chloride self-exchange flux by phloretin was reduced at low pH. Our interpretation of these findings is that the carrier-mediated flux becomes a progressively smaller fraction of the total flux at lower pH values and that a different transport mode requiring two chloride ions to form the permeant species and having a low specificity and temperature dependence becomes significant below pH5. A possible mechanism for this transport is that chloride crosses red cell membranes as dimers of HCl at these very low pH values.


Mar 16, 1973·Biochimica Et Biophysica Acta·S Lepke, H Passow
Apr 1, 1970·Die Naturwissenschaften·B Deuticke
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Oct 5, 1979·The Journal of Membrane Biology·A L Obaid, E D Crandall
Jan 1, 1980·The Journal of Membrane Biology·A L ObaidE D Crandall
Jan 1, 1976·The Journal of Membrane Biology·R B Gunn, R G Kirk
Jul 3, 2009·The Journal of Membrane Biology·Yuliya V KucherenkoFlorian Lang
Jan 1, 1996·The Journal of General Physiology·M L Jennings, M F Adame
Jun 1, 1976·The Journal of General Physiology·O S AndersenA Cass
Sep 1, 1979·The Journal of General Physiology·V CastranovaJ F Hoffman
Jan 1, 1980·Annals of the New York Academy of Sciences·J O WiethJ Funder
Jan 1, 1980·Annals of the New York Academy of Sciences·R B Gunn, O Fröhlich
Feb 11, 2000·Japanese Journal of Pharmacology·T IshibashiM Nishio
Jul 30, 1985·Biochemistry·B CampbellR F Steiner
Apr 17, 2013·Physiological Reviews·Mark D Parker, Walter F Boron
Jan 1, 1975·The Journal of Membrane Biology·R B Gunn, J A Cooper
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·F A RecchiaT H Hintze
Aug 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·D Schubert, E Klappauf
Aug 4, 1998·The American Journal of Physiology·L M MaglovaJ M Russell
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Related Concepts

Cell Membrane Permeability
Chloride Ion Level
Hydrogen-Ion Concentration

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