Some effects of the venom of the Chilean spider Latrodectus mactans on endogenous ion-currents of Xenopus laevis oocytes

Biochemical and Biophysical Research Communications
Jorge ParodiAtaúlfo Martínez-Torres

Abstract

A study was made of the effects of the venom of the Chilean spider Latrodectus mactans on endogenous ion-currents of Xenopus laevis oocytes. 1 microg/ml of the venom made the resting plasma membrane potential more negative in cells voltage-clamped at -60 mV. The effect was potentially due to the closure of one or several conductances that were investigated further. Thus, we determined the effects of the venom on the following endogenous ionic-currents: (a) voltage-activated potassium currents, (b) voltage-activated chloride-currents, and (c) calcium-dependent chloride-currents (Tout). The results suggest that the venom exerts its action mainly on a transient outward potassium-current that is probably mediated by a Kv channel homologous to shaker. Consistent with the electrophysiological evidence we detected the expression of the mRNA coding for xKv1.1 in the oocytes.

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Citations

Feb 27, 2013·Pflügers Archiv : European journal of physiology·L D Ochoa-de la PazA Martinez-Torres
Feb 23, 2010·Systems Biology in Reproductive Medicine·Jorge ParodiFernando Romero Mejías
May 1, 2019·International Journal of Molecular Sciences·Amani CheikhRym Benkhalifa
Feb 4, 2010·Methods : a Companion to Methods in Enzymology·Jan TerhagMichael Hollmann

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