PMID: 702517Sep 18, 1978Paper

Some effects of trinitrocresolate and valinomycin on Na and K transport across thin lipid bilayer membranes: a steady-state analysis with simultaneous tracer and electrical measurements

The Journal of Membrane Biology
H GinsburgD C Tosteson

Abstract

This paper describes the effect of trinitrocresolate anions (TNC-) on the electrical conductance (Gm), and tracer-measured unidirectional Na and K fluxes (MNa and MK) across bilayers formed from sheep red cell lipids dissolved in decane. In the absence of TNC-, typical low conductances were observed, while the cation fluxes were too low to measure by our techniques (less than 10(-12) moles cm-2 sec-1). In the presence of TNC- (10(-2) M), Gm increased and TNC- was the main charge carrier in the system. The cationic fluxes were also much increased, but the membranes showed no significant selectivity between K and Na. Furthermore, the Na and K fluxes were at least two orders of magnitude larger than the ionic fluxes calculated from Gm. Thus, almost all of the K and Na transport across the membrane in the presence of TNC- is electrically silent and is probably carried out as KTNC and NaTNC ion pairs. In the presence of valinomycin (10(-6) M) and no TNC-, both the ion fluxes and Gm were 10(3) times larger in KCl than in NaCl, thus exhibiting the characteristic high selectivity of valinomycin for K over Na. In the presence of both valinomycin (10(-6) M) and TNC- (10(-2) M), this selectivity disappeared in that both Gm and MNa in the ...Continue Reading

References

Jun 1, 1976·The Journal of General Physiology·O S AndersenA Cass
May 1, 1971·The Journal of General Physiology·R B Gunn, D C Tosteson
Dec 1, 1967·The Journal of General Physiology·T E AndreoliD C Tosteson
Feb 21, 1967·Biochemical and Biophysical Research Communications·P Mueller, D O Rudin
Jul 1, 1967·The Journal of General Physiology·T E AndreoliD C Tosteson

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Citations

Oct 31, 1980·The Journal of Membrane Biology·V C Chiu, D H Haynes
May 28, 1979·Biochemical and Biophysical Research Communications·R Panet, H Atlan
Aug 3, 2011·Journal of Colloid and Interface Science·N AnithaM Palaniandavar

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