PMID: 326970Jan 1, 1977Paper

Some methodologic aspects of the chromium chloride method for coupling antigen to erythrocytes

Journal of Immunological Methods
R Kofler, G Wick

Abstract

The influence of various methodological variables on the CrCl3 technique for coupling antigen to the surface of erythrocytes has been investigated. Tests should be performed in protein- and phosphate-free medium. The CrCl3 stock solution should be stored at a pH of 5.0 and working dilultions prepared in acetate buffer (pH 5.5). The coupling procedure itself was performed as described by Goding (1976) with slight modifications. The relation between antigen and CrCl3 concentrations was found to be of crucial importance as excess of antigen inactivates CrCl3 whereas lack of antigen or excess of CrCl3, leads to spontaneous agglutination. A preliminary test based on determination of the minimum concentration of CrCl3 which produces spontaneous agglutination in the absence of antigen, and coupling if antigen is available, can be employed to predict the optimal concentration with sheep, human or chicken red blood cells. The pH-dependency of the coupling process is emphasized.

Citations

Jan 1, 1978·Journal of Immunological Methods·J W Goding
Jan 1, 1979·Journal of Immunological Methods·H WolfG Wick
Jan 1, 1981·Journal of Immunological Methods·G F Burns, B L Pike
Feb 25, 1983·Journal of Immunological Methods·B J WeillP H Schur
Jul 29, 1983·Journal of Immunological Methods·L M Karavodin, S H Golub
Mar 16, 1984·Journal of Immunological Methods·J WormmeesterK De Groot
Jan 1, 1985·Developmental and Comparative Immunology·W C GauseJ A Marsh
Jul 1, 1984·Veterinary Immunology and Immunopathology·P J FelsburgP F Jezyk
Aug 24, 1987·Journal of Immunological Methods·M K LoftagerV Andersen
Nov 15, 2007·The Journal of Biological Chemistry·Yuan-Hung ChienDeborah Leckband
Sep 1, 1993·Journal of Immunoassay·L Kittigul, K Ratanabanangkoon
Feb 1, 1980·The Journal of Experimental Medicine·A N TheofilopoulosF J Dixon
Jun 1, 1979·Journal of Immunogenetics·G WickK Hála
Feb 1, 1982·Archives of Disease in Childhood·S A Roberts, J F Soothill
Apr 17, 2012·Journal of Cell Science·Hamid TabdiliDeborah E Leckband
Jun 22, 2012·Journal of Cell Science·Hamid TabdiliDeborah Leckband
Mar 12, 2009·Science in China. Series C, Life Sciences·Fan YangMian Long
Apr 11, 2006·Biophysical Journal·Mian LongCheng Zhu
Feb 20, 2007·Analytical Biochemistry·Ben W MuirN Joe Maeji
Sep 22, 2016·Journal of Immunological Methods·Nicholas G WelchBenjamin W Muir
Sep 21, 2016·Analytical Chemistry·Nicholas G WelchBenjamin W Muir
May 1, 1986·European Journal of Immunology·H M Etlinger
Feb 7, 2019·American Journal of Physiology. Cell Physiology·Shuang PengMian Long
May 1, 1977·In Vitro·R B Edwards
Jul 16, 2015·The Journal of Biological Chemistry·Nitesh ShashikanthDeborah E Leckband

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.