PMID: 601804Nov 1, 1977Paper

Some observations on glass-knife making

Stain Technology
R T Ward

Abstract

The yield of usable knife edge per knife (for thin sectioning) was markedly increased when glass knives were made at an included angle of 55 degrees rather than the customary 45 degrees. A large number of measurements of edge check marks made with a routine light scattering method as well as observations made on a smaller number of test sections with the electron microscope indicated the superiority of 55 degrees knives. Knives were made with both taped pliers and an LKB Knifemaker. Knives were graded by methods easily applied in any biological electron microscope laboratory. Depending on the mode of fracture, the yield of knives having more than 33% of their edges free of check marks was 30 to 100 times greater at 55 degrees than 45 degrees.

References

Jul 25, 1956·The Journal of Biophysical and Biochemical Cytology·D A CAMERON
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·S WEINER
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT
Jun 1, 1962·Journal of Ultrastructure Research·J ANDRE
Oct 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·B BYERS, K R PORTER
Jun 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·H LATTA, J F HARTMANN

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Citations

May 1, 1997·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·W WangK Du
May 1, 1983·Stain Technology·D R DisharoonF H Lofftus
Feb 1, 1984·Journal of Microscopy·P R Richards
Nov 1, 1980·Journal of Microscopy·L L Franchi, D Symons

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