Some properties and action mode of (1-->4)-alpha-L-guluronan lyase from Enterobacter cloacae M-1

Carbohydrate Research
T ShimokawaH Kobayashi

Abstract

An intracellular alginate lyase was purified from Enterobacter cloacae M-1 by successive fractionation on Q Sepharose FF, SP Sepharose FF, and Sephacryl S-200 HR. The purified enzyme gave a single band on SDS-PAGE and isoelectric focusing. The enzyme easily degraded polyguluronate and produced unsaturated oligoguluronic acids with a wide range of dp. The major end product of the enzyme reaction on polyguluronate was unsaturated triuronic acid. The pattern of oligoguluronic acids (dp 2-9) generated with the enzyme was investigated by fluorophore-assisted carbohydrate electrophoresis. The enzyme was not capable of degrading oligoguluronic acids having dp < 4. The degradation rate of heptaguluronic acid by this enzyme remarkably increased, compared with that of hexaguluronic acid, and heptaguluronic acid had a single preferential point of cleavage by this enzyme. On the basis of the cleavage pattern of oligoguluronic acids, the number of subsites was estimated to be seven for this enzyme. The catalytic site of the enzyme is located between the second and the third subsites from the non-reducing end.

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Citations

Nov 11, 2011·Journal of Industrial Microbiology & Biotechnology·Su-Jung SimDuwoon Kim
Mar 1, 2006·Journal of the American Society for Mass Spectrometry·Zhenqing ZhangWengang Chai
Jan 26, 2017·International Journal of Biological Macromolecules·Bidyut R Mohapatra
Sep 6, 2006·Acta Biochimica Et Biophysica Sinica·Yuan-Hong WangWei-Shang Ji

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