Some properties of a macromolecular conjugate of lysozyme prepared by modification with a monomethoxypolyethylene glycol derivative

Bioscience, Biotechnology, and Biochemistry
Yuichi Nodake, N Yamasaki

Abstract

Hen egg-white lysozyme was modified with a succinyl ester derivative of monomethoxypolyethylene glycol (mPEG-COONSu), and some properties of the resulting conjugate (mPEG-lysozyme) were studied. The conjugate was prepared by modification of lysozyme with mPEG-COONSu and purified with use of columns of CM-Toyopearl 650M and Sephadex G-75. Analytical data indicated that in the conjugate, 1.05 moles of mPEG with an average molecular weight of 5,000 were covalently attached to the lysozyme molecule. Tryptic peptide analysis of the conjugate showed that Lys 33 in lysozyme is the residue mainly modified with mPEG-COONSu. Covalent attachment of the mPEG-derivative to amino groups greatly increased the thermostability of lysozyme without any conformational change of the protein molecule. mPEG-lysozyme retained full enzyme activity for glycol chitin, but lytic activity for Micrococcus luteus cells in neutral media was 75% of that of native lysozyme and its optimal pH was at pH 5.0. It was also found that the reactivity of lysozyme with anti-lysozyme antibody from BALB/c mice or human lymphocytes was decreased by modification with mPEG-COONSu. From these findings, it was suggested that mPEG-COONSu can be advantageously used for protein t...Continue Reading

References

Mar 5, 1987·The New England Journal of Medicine·M S HershfieldA L Kobayashi
Jul 8, 1985·FEBS Letters·B VeerapandianM Vijayan
Apr 18, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C C BlakeV R Sarma
Sep 1, 1971·The Biochemical Journal·R Fields
Jul 31, 1984·Biochemical and Biophysical Research Communications·Y InadaY Saito
May 1, 1952·Journal of Bacteriology·A N SMOLELIS, S E HARTSELL

Citations

Apr 10, 2010·Bioscience, Biotechnology, and Biochemistry·Yuichi NodakeNobuyuki Yamasaki
Jul 22, 2010·Pharmaceutical Biology·Débora da Silva Freitas, José Abrahão-Neto
Aug 21, 2014·Journal of Pharmaceutical Sciences·Louise Stenstrup HolmMarco van de Weert
May 19, 2009·International Journal of Pharmaceutics·Taishi HigashiKaneto Uekama
Sep 12, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Benjamin MaiserJürgen Hubbuch
Sep 1, 2016·Current Opinion in Chemical Biology·Paul B Lawrence, Joshua L Price
Aug 1, 2015·PloS One·Louise Stenstrup HolmMarco van de Weert
Jun 23, 2015·Macromolecular Bioscience·Parker LeeJonathan Pokorski
May 31, 2002·Bioscience, Biotechnology, and Biochemistry·Ismael D BiancoGerardo D Fidelio
Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shinji KatsuraEtsuo Yonemochi

Related Concepts

Monomethoxypolyoxyethylene
Circular Dichroism, Vibrational
Enzyme Stability
Heating
Mice, Inbred BALB C
Leftose
Vigilon
Fluorescence Spectroscopy

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