Some properties of heat-resistant and heat-sensitive strains of Clostridium perfringens. I. Heat resistance and toxigenicity.

Journal of Bacteriology
K F Weiss, D H Strong

Abstract

Heat resistance at 100 C (D-values), sporulating ratios, toxigenicity for mice, and lecithinase activity (as micrograms per milliliter of enzyme, ascertained by the lecithovitellin reaction) were determined for four strains of Clostridium perfringens. A definite inverse relationship between thermal resistance and toxigenicity was found. The D-values ranged from 17.6 for the most heat-resistant strain to 0.3 for the strain possessing the least heat resistance, with corresponding lecithinase activities from 25 to 133 mug/ml of enzyme. The sporulating ratios did not differ greatly between the strains. The heat stability of the toxin was greater at 100 C than at 75 C. There was a noticeable difference between the heat stabilities of the toxin in the culture fluids of the heat-sensitive and heat-resistant strains at pH 7.0 when the toxic filtrates were held at 100 C. At a holding temperature of 75 C, a similar but lesser difference was observed at pH 5.5. Heat resistance and lecithinase activity did not change when a substrain of the least heat-resistant parent strain was obtained through heat selection by a single transfer, or when the most heat-resistant strain was transferred serially 12 times.

References

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Citations

Mar 1, 1969·The Journal of Hygiene·M NakamuraW R Cross
Sep 1, 1967·The Journal of Hygiene·M Nakamura, J D Converse
Dec 9, 2009·Molecular Microbiology·Benjamin C OrsburnDavid L Popham
Mar 1, 1968·The Journal of Applied Bacteriology·T A Roberts
Jan 1, 1985·Microbiology and Immunology·S NakamuraS Nishida
May 1, 1968·Journal of Bacteriology·C L DuncanD H Strong
Apr 1, 1972·Journal of Bacteriology·C L DuncanM Sebald
Mar 1, 1969·Bacteriological Reviews·P Schaeffer
Dec 1, 1970·Applied Microbiology·S M Harmon, D A Kautter

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