Jun 1, 1976

Some properties of human platelet monoamine oxidase in iron-deficiency anaemia

Clinical Science and Molecular Medicine
M B YoudimH F Woods

Abstract

1. Monoamine oxidase activity in platelets prepared from the blood of patients with iron-deficiency anaemia was significantly lowered when compared with that in platelets from normal subjects. 2. The Km values of the platelet enzyme for the substrates dopamine, 5-hydroxytryptamine, phenylethylamine and kynuramine were similar for the platelet enzyme from iron-deficient and normal groups. 3. Heat-in-activation studies showed that the platelet monoamine oxidase from iron-deficient subjects was more labile to this treatment, when compared with the platelet enzyme from normal subjects. 4. The sensitivity of platelet monoamine oxidase to the inhibitors, clorgyline and deprenil, was increased in iron-deficiency anaemia. 5. Binding studies with the 14C-binding irreversible monoamine oxidase inhibitor, deprenil, showed that the amount of enzyme capable of binding this inhibitor was lowered by 48% in platelets from iron-deficient patients when compared with platelets from normal subjects. 6. The results show that there is a lowered amount of active enzyme in platelets from iron-deficient subjects. It is suggested that iron is necessary either for the synthesis of monoamine oxidase apoenzyme or is a cofactor for an enzyme which attaches ...Continue Reading

  • References
  • Citations7

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations7

Citations

Mentioned in this Paper

Apoenzymes
Serotonin
Monoamine Oxidase [PK]
Iron Deficiency Anemia
Monoamine Oxidase Inhibitors
Plasma Protein Binding Capacity
Monoamine Oxidase
Kynuramine
RIMA (Reversible Inhibitor of Monoamine Oxidase A)
Monoamine Oxidase Inhibitor [EPC]

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.