PMID: 7016101Feb 1, 1981Paper

Some properties of neutral proteinases from lysosomes of rabbit polymorphonuclear leucocytes

The Australian Journal of Experimental Biology and Medical Science
M L Britz, D A Lowther

Abstract

Neutral proteinases capable of degrading proteoglycan were found in lysosomes of rabbit polymorphonuclear leucocytes extracted with 0 . 01 M citric acid. Esterase activity against an elastase substrate was also present but chymotrypsin- and trypsin-like activities were not detected; azocasein-degrading activity was poor. Proteoglycanase activity was stimulated by high concentrations of salts (0 . 2 M KCl) and divalent cations (Ca, Mg, Mn, Zn) but was inhibited by Cu++. Elastase activity was also stimulated by high ionic strength buffers and KCl, but not as much by divalent cations, and was inhibited by Cu++. Proteoglycanase in crude extracts was inhibited by EDTA, phenylmethanesulphonylfluoride (Pms-F), cell cytosol, alpha 1-antitrypsin, gold thiomalate and N-acetyl-di-L-alanyl-L-propyl-L-valine chloromethyl ketone (AAAPVCK). Partial inhibition by N-alpha-p-tosyl-L-lysine chloromethyl ketone (TLCK) and L-l-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) occurred. Elastase adsorbed to CM-cellulose and was eluted by 0 . 6-0 . 7 M NaCl; a metallo-proteinase failed to adsorb completely but was retarded by the CM-cellulose. Isoelectric focusing showed that the major proteinases had pI's of 5 . 5, 8 . 5 and 9 . 1; the activity wi...Continue Reading

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