PMID: 10068Sep 1, 1976

Some properties of p-coumarate decarboxylase from Cladosporium phlei

Canadian Journal of Microbiology
T Harada, Y Mino


The optimal pH and temperature of p-coumarate decarboxylase were 6.0 and 23 degrees C respectively. The enzyme activity was reduced to three quarters by heat treatment at 35 degrees C for 5 min and by half at 25 degrees C in 24 h, but kept almost unchanged at -20 degrees C at least for 10 days. The activity was not inhibited by potassium cyanide, sodium diethyldithiocarbamate, ethylenediaminetetraacetic acid disodium salt, or sodium citrate at 10 mM concentration, but was inhibited by p-chloromercuribenzoate or iodoacetate at 0.1 mM, the inhibition by the former being prevented to a great extent by the presence of reduced glutathione or dithiothreitol. The activity was inhibited by maleic acid cinnamic acid, or p-methoxycinnamic acid, but not by fumaric acid, acrylic acid, p-hydroxystyrene, furcatin p-hydroxyphenylacetic acid, or phloretic acid. An unsubstituted p-hydroxy group on the benzene ring and an acrylic acid side chain were required for the enzyme activity. Km value for trans-p-coumaric acid was about 6.5 X 10(-4) M.


Dec 1, 1995·Journal of Industrial Microbiology·J P RosazzaB Rousseau
Mar 25, 2010·Journal of Industrial Microbiology & Biotechnology·José María LandeteRosario Muñoz
Jul 21, 2012·Studies in Mycology·K BenschP W Crous

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Carbon Dioxide
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