Jan 1, 1975

Some properties of rat myocardial ornithine decarboxylase and the in vitro effects of nucleotides

Recent Advances in Studies on Cardiac Structure and Metabolism
K GibsonP Harris

Abstract

Myocardial ornithine decarboxylase appears to have characteristics similar to those of enzymes isolated from other tissues. Ornithine decarboxylase activity decreased very rapidly after the death of the animal. Storage of the cell sap fraction at 0 degrees C or -15 degrees C, however, led to only a small decrease in the enzyme activity up to 3 days after preparation. Pyridoxal phosphate at an optimum of 50 muM was essential for full enzyme activity. Thiol compounds did not increase the myocardial ornithine decarboxylase enzyme activity. The subcellular distribution of the enzyme in the myocardium was found to be different from that reported in other tissues. A partial purification of the enzyme was possible using the proteins precipitated at pH 5 from a cell-soluble fraction or by passing a soluble fraction through a Sephadex G 100 gel column. ATP, ADP, and AMP inhibited ornithine decarboxylase at high concentrations (5 mM), but GTP, CTP, and ITP inhibited at a 1 mM concentration and above.

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Mentioned in this Paper

Ribonucleotides
Enzymes, antithrombotic
Myocardium
Pyridoxal Phosphate
Sulfhydryl Compounds
Carboxy-Lyases
Enzyme Activity
Enzymes for Treatment of Wounds and Ulcers
Cyclic AMP, (R)-Isomer
Blood Enzyme Activity (Lab Test)

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