PMID: 2493252Feb 1, 1989Paper

Some recombinant human cytokines stimulate glycosaminoglycan synthesis in human synovial fibroblast cultures and inhibit it in human articular cartilage cultures

Arthritis and Rheumatism
I YaronM Yaron

Abstract

Recombinant human cytokines were compared for their effects on glycosaminoglycan (GAG) synthesis in human synovial fibroblast cultures and human articular cartilage explant cultures. In fibroblast cultures, recombinant human interleukin-1 alpha (rHuIL-1 alpha), rHuIL-1 beta, and recombinant human tumor necrosis factor alpha (rHuTNF alpha) stimulated hyaluronic acid (HA) production and, to a lesser extent, sulfated GAG production, while recombinant human gamma-interferon did not have a significant effect. Half-maximal stimulation of HA by rHuIL-1 beta was 0.14 pM, while stimulation for rHuIL-1 alpha and rHuTNF alpha was 1.6 pM and 32 pM, respectively. Indomethacin (10 micrograms/ml) had no influence on HA stimulation by cytokines, while hydrocortisone (2-10 micrograms/ml) caused a significant reduction. In articular cartilage cultures, the cytokines inhibited production of sulfated GAGs. The activity of rHuIL-1 beta was greater than that of rHuIL-1 alpha (half-maximal inhibition at 0.71 pM and 4.7 pM, respectively) and both were considerably more active than rHuTNF alpha; gamma-interferon again had no significant effect. Neither indomethacin nor hydrocortisone influenced cytokine-induced inhibition by either rHuIL-1 preparation....Continue Reading

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Citations

Mar 21, 2000·Journal of Cellular Biochemistry·E Tufvesson, G Westergren-Thorsson
May 15, 1993·Experientia·J J Nietfeld
Jan 1, 1992·Biotherapy·B VivianA J Trevis
Jan 1, 1997·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·X Chevalier
May 26, 1999·Annals of the Rheumatic Diseases·D M ChangM L Chang
Dec 1, 1994·The Journal of Clinical Investigation·M B GoldringJ F Apperley
Aug 1, 1995·Immunopharmacology and Immunotoxicology·D M Chang
Jun 1, 1992·Baillière's Clinical Rheumatology·J M Dayer, H Fenner
Nov 2, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bruno C MenarimLinda A Dahlgren
Mar 1, 1990·Arthritis and Rheumatism·W P Arend, J M Dayer
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Monica Helena Monteiro do NascimentoDaniele Ribeiro de Araujo

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