Sonic Hedgehog mediator Gli2 regulates bladder mesenchymal patterning

The Journal of Urology
Wei ChengPeter C W Kim

Abstract

Congenital bladder anomalies are a major challenge to pediatric urologists. Understanding the mechanism of bladder development is crucial for advancing patient treatment. Current evidence suggests that Shh (R&D Systems) is an epithelial signal regulating bladder development, although the mechanism of the regulation is still unclear. We examined the regulation of bladder mesenchymal development. Mutation analysis, immunohistochemistry, immunoblot, in situ hybridization, and primary cell culture and transfection were performed. The mesenchyma proximal to the epithelium was defined as the inner zone and that distal to the epithelium was defined as the outer zone. We found that the Shh transcriptional factor Gli2 and the Shh target gene Bmp4 (R&D Systems) were expressed in the inner mesenchymal zone of the bladder, where active cell proliferation was observed. In Gli2(-/-) bladder primary mesenchymal cell cultures transfection with adenoviruses expressing DeltaNGli2, a constitutionally active form of Gli2, up-regulated Bmp4 expression and promoted cell proliferation. In the outer mesenchymal zone, where Gli2 and Bmp4 expression was not detectable, smooth muscle alpha-actin was expressed. In Gli2(-/-) embryo bladders Bmp4 expression...Continue Reading

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Citations

Jun 8, 2013·Journal of Pediatric Urology·Istiak MahfuzWei Cheng
Jan 14, 2016·Current Urology Reports·Islam S SyedWalid A Farhat
Dec 12, 2012·Journal of Pediatric Surgery·Rui LiangWei Cheng
Jun 15, 2010·Differentiation; Research in Biological Diversity·Gregory TasianLaurence Baskin
Mar 17, 2010·Differentiation; Research in Biological Diversity·Mei CaoLaurence Baskin
Feb 15, 2013·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Hila Milo Rasouly, Weining Lu
Aug 2, 2011·Developmental Biology·Sheila M BellJeffrey A Whitsett
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Jan 22, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Caihong WangIndira U Mysorekar
Apr 18, 2018·Lower Urinary Tract Symptoms·Sandra FreseAlina C Hilger
Jan 6, 2017·American Journal of Physiology. Renal Physiology·Y IkedaC M Bates
Feb 14, 2019·Cells·Paweł NiewiadomskiKatarzyna Chojnowska
Nov 6, 2009·Nature Reviews. Urology·Karen J Aitken, Darius J Bägli
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Jul 11, 2020·Nature Reviews. Urology·Ashley R JacksonBrian Becknell
May 15, 2015·Development·Kylie M GeorgasCathy Mendelsohn
Sep 7, 2021·Stem Cells and Development·Monika BuhlMarta Pokrywczynska

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