Sonic hedgehog pathway genes are expressed and transcribed in the adult mouse epididymis

Journal of Andrology
Terry T TurnerJason P Jacobs

Abstract

One role of the hedgehog (hh) signaling pathway during development is to assist in establishing pattern orientation in the embryo. The structure and function in the adult epididymis is highly patterned, and since the sonic hedgehog (Shh) pathway is known to be functional in the developing male tract and the expression of other pattern-influencing genes has recently been found in the adult epididymis, we have examined the adult mouse epididymis for Shh pathway molecules. Examination was at both the gene and protein level. Shh, the secreted signal molecule, patched (Ptc), its membrane receptor, and Gli-1, a downstream transcription factor, were detected at the gene level with semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and at the protein level with Western blot analysis. Immunohistochemical localization further detected Shh specifically in the epididymal epithelium. It was hypothesized that efferent duct ligation (EDL) would alter epididymal segmentation within 30 days of the ligation, especially in the proximal segments of the caput epididymis. It was further hypothesized that these alterations would be correlated with changes in the expression of genes in the Shh pathway. EDL did not alter epididym...Continue Reading

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Citations

Nov 23, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Heather L Franco, Humphrey H-C Yao
May 28, 2019·Andrology·Laura GirardetClémence Belleannée
Apr 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura GirardetClémence Belleannée
Apr 30, 2019·Andrology·L PinelD G Cyr
Nov 4, 2011·The Journal of Biological Chemistry·Shu-Chun ChangJohn R Couchman
Jan 27, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Fernando F MigoneSusan M Quirk
Nov 24, 2011·Development·Jirouta KitagakiAlan O Perantoni
Feb 12, 2008·Developmental Biology·Donald J GlotzerBjorn R Olsen

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