Sonic Hedgehog regulates thymic epithelial cell differentiation

Journal of Autoimmunity
José Ignacio SaldañaTessa Crompton

Abstract

Sonic Hedgehog (Shh) is expressed in the thymus, where it regulates T cell development. Here we investigated the influence of Shh on thymic epithelial cell (TEC) development. Components of the Hedgehog (Hh) signalling pathway were expressed by TEC, and use of a Gli Binding Site-green fluorescence protein (GFP) transgenic reporter mouse demonstrated active Hh-dependent transcription in TEC in the foetal and adult thymus. Analysis of Shh-deficient foetal thymus organ cultures (FTOC) showed that Shh is required for normal TEC differentiation. Shh-deficient foetal thymus contained fewer TEC than wild type (WT), the proportion of medullary TEC was reduced relative to cortical TEC, and cell surface expression of MHC Class II molecules was increased on both cortical and medullary TEC populations. In contrast, the Gli3-deficient thymus, which shows increased Hh-dependent transcription in thymic stroma, had increased numbers of TEC, but decreased cell surface expression of MHC Class II molecules on both cortical and medullary TEC. Neutralisation of endogenous Hh proteins in WT FTOC led to a reduction in TEC numbers, and in the proportion of mature Aire-expressing medullary TEC, but an increase in cell surface expression of MHC Class II ...Continue Reading

References

Dec 22, 1998·The Journal of Experimental Medicine·H S AzzamP E Love
Jul 18, 2002·Immunity·Andrea R BennettC Clare Blackburn
Oct 21, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rosa SacedónAngeles Vicente
Feb 2, 2006·Immunological Reviews·Georg HolländerYousuke Takahama
Mar 7, 2006·Nature Immunology·Abdeljabar El AndaloussiIannis Aifantis
May 16, 2007·The Journal of Experimental Medicine·Simona W RossiGraham Anderson
Aug 11, 2007·Nature Reviews. Immunology·Tessa CromptonAriadne L Hager-Theodorides
Nov 22, 2011·European Journal of Immunology·Anna L FurmanskiTessa Crompton
May 18, 2012·Trends in Immunology·Graham Anderson, Yousuke Takahama
Aug 23, 2012·PloS One·Sunil BadvePatrick J Loehrer
Jan 10, 2013·European Journal of Immunology·Song BaikWilliam E Jenkinson
May 21, 2013·Autoimmunity Reviews·Gerhild Wildner, Ulrike Kaufmann
Oct 11, 2013·Frontiers in Immunology·T Petteri Arstila, Hanna Jarva
Nov 12, 2013·European Journal of Immunology·Nuno L AlvesWilliam E Jenkinson
Feb 1, 2014·European Journal of Immunology·Marco CarbonePietro Invernizzi
Apr 15, 2014·Nature Immunology·Mathias Hauri-HohlSteven F Ziegler
Aug 26, 2014·Nature Cell Biology·Nicholas BredenkampCatherine Clare Blackburn
Dec 21, 2014·Haematologica·Antonis DagklisJan Cools
Mar 19, 2015·Frontiers in Immunology·Olga Ucar, Kristin Rattay
Jul 5, 2015·Journal of Autoimmunity·Jill A Hollenbach, Jorge R Oksenberg
Aug 8, 2015·Journal of Autoimmunity·G J WebbG M Hirschfield

❮ Previous
Next ❯

Citations

Feb 23, 2017·Annual Review of Immunology·Jakub Abramson, Graham Anderson
May 24, 2017·The Journal of Experimental Medicine·Anisha SolankiTessa Crompton
Aug 10, 2016·Journal of Developmental Biology·Alessandro BarbaruloTessa Crompton
Aug 9, 2017·Clinical Reviews in Allergy & Immunology·Junmin SongPatrick S C Leung
Oct 14, 2017·Organogenesis·Sara Montero-HerradónAgustín G Zapata
Jul 3, 2019·The Journal of Clinical Investigation·Eleftheria PapaioannouTessa Crompton
Feb 23, 2020·Frontiers in Immunology·Hong-Xia WangYu-Rong Qiu
Aug 6, 2019·Frontiers in Immunology·Konstantinos MengrelisTessa Crompton
Feb 6, 2017·The Journal of Experimental Medicine·Stefano VolpiLuigi D Notarangelo
Oct 26, 2021·The Journal of Allergy and Clinical Immunology·Rafael Gras-PeñaMegan Sykes

❮ Previous
Next ❯

Methods Mentioned

BETA
FCS
mechanical dissociation
PCR
transgenic
flow cytometry

Software Mentioned

affy
R
Flowjo

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.