Abstract
Sonic Hedgehog (Shh), a member of hedgehog peptides family, is expressed in gastric gland epithelium. To elucidate Shh function to gastric mucosal cells, we examined the effect of Shh on the proliferation of a rat normal gastric mucosal cell line, RGM-1. RGM-1 cells express essential components of Shh receptor system, patched-1, and smoothened. Shh enhanced DNA synthesis in RGM-1 cells and elevated intracellular calcium concentration ([Ca2+]i). In addition, Shh as well as calcium ionophore A32187 rapidly activated ERK. However, Shh failed to activate ERK under calcium-free culture condition. Pretreatment of cells with PD98059 attenuated the DNA synthesis promoted by Shh. Moreover, when cells were pretreated with cyclopamine, Shh could not elevate [Ca2+]i, activate ERK or promote DNA synthesis. On the other hand, although Shh induced Gli-1 nuclear accumulation in RGM-1 cells, Shh activated ERK even in cells pretreated with actinomycin D. These results indicate that Shh promotes the proliferation of RGM-1 cells through an intracellular calcium- and ERK-dependent but transcription-independent pathway via Patched/Smoothened receptor system.
References
Dec 1, 1995·Genes & Development·A E MünsterbergA B Lassar
May 1, 1996·In Vitro Cellular & Developmental Biology. Animal·I KobayashiT Kamada
Nov 22, 1997·Current Biology : CB·A ApelqvistH Edlund
Jan 31, 1998·The Journal of Clinical Investigation·H OhnishiJ A Williams
Sep 10, 1998·Nature Genetics·Y LitingtungC Chiang
Feb 18, 1999·Current Biology : CB·M MuroneF J de Sauvage
Jun 3, 2000·The Journal of Biological Chemistry·D L DeCampM R Lerner
Feb 13, 2001·The Journal of Clinical Investigation·J SuzukiT Fujita
Mar 29, 2001·Nature Immunology·G BhardwajM Bhatia
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·J XieF McCormick
Aug 7, 2001·Gastroenterology·G R van den BrinkM P Peppelenbosch
Sep 12, 2001·The Journal of Biological Chemistry·C KanamaruT Fujita
Dec 4, 2001·Genes & Development·P W Ingham, A P McMahon
Dec 4, 2001·Pharmacology·Yasuhiro TsukimiSusumu Okabe
Aug 23, 2002·Nature·J TaipaleP A Beachy
Aug 30, 2002·Current Opinion in Genetics & Development·Kent Nybakken, Norbert Perrimon
Oct 16, 2002·Gut·G R van den BrinkM P Peppelenbosch
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·James K ChenPhilip A Beachy
Dec 23, 2003·The Journal of Biological Chemistry·Hirohide OhnishiKentaro Sugano
Aug 18, 2004·Biochemical Pharmacology·D N Watkins, C D Peacock
Sep 1, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·A Hurtado-LorenzoP R Lowenstein
Citations
Nov 28, 2006·Nature Reviews. Cancer·Susan A WatsonArjun Takhar
Jul 2, 2009·Reproduction, Fertility, and Development·Ngoc Tan NguyenJyh-Cherng Ju
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yesser H Belgacem, Laura N Borodinsky
Aug 16, 2006·Molecular and Cellular Biology·Alison R MeloniMarc G Caron
Aug 18, 2006·BMC Biology·Lucy J LeiperJ Martin Collinson
Apr 28, 2009·Genome Biology·Reuben ThomasChristopher J Portier
Jun 14, 2013·PloS One·Luciana H Osaki, Patricia Gama
Oct 15, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·A Denise R GarciaAlexandra L Joyner
Nov 4, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dan ZhangJonathan S Marchant
Apr 8, 2011·Molecular Medicine Reports·Ling YangJingwu Xie
May 4, 2010·Digestive Diseases and Sciences·Jason MartinYana Zavros
Feb 4, 2014·Nutrition·Heloisa GhizoniPatrícia Gama
Jul 29, 2010·Theriogenology·Ngoc Tan NguyenJyh-Cherng Ju
Dec 24, 2015·Neurochemical Research·Hiroaki OkudaAkio Wanaka
Feb 3, 2009·Journal of Neurochemistry·Peter J AtkinsonWarren D Hirst
Sep 29, 2007·Stem Cells·Jung Sun HeoHo Jae Han
Mar 28, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jason R SpenceKatia Del Rio-Tsonis
Nov 15, 2013·Journal of Gastroenterology and Hepatology·Takeshi TanakaOsamu Yokosuka
Aug 21, 2015·Seminars in Cancer Biology·Elisabetta Rovida, Barbara Stecca
Aug 11, 2007·Biochimica Et Biophysica Acta·Dafna EliaOrna Halevy
Oct 30, 2016·Biochemical and Biophysical Research Communications·Hankyu Lee, Hyuk Wan Ko
Mar 24, 2016·PloS One·Tsuyoshi ShimoAkira Sasaki
May 15, 2013·International Journal of Molecular Sciences·Luciana H Osaki, Patrícia Gama
Feb 18, 2020·Expert Review of Gastroenterology & Hepatology·Huiqin GaoWensheng Pan
Dec 8, 2017·Journal of Cellular Physiology·Sanghoon LeeByeong Chun Lee
Jan 20, 2021·Neurochemical Research·Masanori HijiokaYoshihisa Kitamura
Oct 7, 2019·Biochemical Pharmacology·Anne VejuxGérard Lizard