Sonicating multi-lumen sliced catheter tips after the roll-plate technique improves the detection of catheter colonization in adults

Journal of Microbiological Methods
María GuembeE Bouza

Abstract

The Maki technique is the standard method for detecting catheter tip (CT) colonization. However, some "multi-lumen" catheters finish in a vaulted fornix and end at different distances from the CT. Therefore, we compared the traditional Maki technique with the sonication method using several cross-cut fragments of the CT. Our objective was to assess the yield of the Maki technique followed by sonication in the detection of adult CT colonization and catheter-related bloodstream infection (C-RBSI). For 3 months, we prospectively performed CT cultures of polyurethane catheters from adult patients admitted to our institution. First, we performed CT culture using the Maki technique on blood agar plates and then sonicated small fragments of CTs in 5 ml of BHI followed by culture of 100 μl of the sonicate. We included a total of 252 CVCs, with overall colonization and C-RBSI rates of 14.3% (36/252) and 5.9% (15/252). Of the 36 colonized CVCs, 21 (58.3%) were detected both by Maki and sonication, 6 (16.7%) were detected only by Maki technique, and 9 (25.0%) only by sonication method. Among 15 episodes with concomitant bacteremia, both techniques were positive and concordant in 9 cases (60.0%), the result of the Maki was positive in only...Continue Reading

References

Jun 9, 1977·The New England Journal of Medicine·D G MakiH W Sarafin
Jan 1, 1992·Diagnostic Microbiology and Infectious Disease·I I RaadR J Sherertz
Jun 10, 2003·Critical Care Medicine·Brian M DobbinsMark H Wilcox
Mar 26, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Emilio BouzaMarta Rodríguez-Créixems
Jun 6, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Leonard A MermelDavid K Warren
May 12, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·María GuembeEmilio Bouza
May 16, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stefan ErbAndreas F Widmer

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Citations

Jan 23, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M GuembeE Bouza
Jun 19, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Beatriz AlonsoMaría Guembe

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