PMID: 9546194Apr 18, 1998Paper

Sonication-dependent electroporation of the erythromycin-producing bacterium Saccharopolyspora erythraea

Applied and Environmental Microbiology
N B FitzgeraldT J Vanden Boom

Abstract

We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques.

References

Sep 1, 1986·The Journal of Antibiotics·H YamamotoC R Hutchinson
Jan 1, 1993·Annual Review of Microbiology·L Katz, S Donadio
Oct 1, 1996·Current Opinion in Biotechnology·C L Hershberger

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Citations

Apr 26, 2013·Beilstein Journal of Organic Chemistry·Stephan KlopriesFrank Schulz
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·D L StassiL Katz
Apr 25, 2012·Applied Microbiology and Biotechnology·Steven BoakesJesús Cortés
Aug 6, 2003·FEMS Microbiology Letters·Sofia StinchiMargherita Sosio

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