Sonication of heart valves detects more bacteria in infective endocarditis

Scientific Reports
Anna GomesBhanu Sinha

Abstract

Optimal antimicrobial treatment of infective endocarditis requires identification and susceptibility patterns of pathogens. Sonication of explanted heart valves could increase the identification and culture of pathogens, as shown in prosthetic joint and pacemaker/ICD infections. We tested 26 explanted heart valves from 20 patients with active definite endocarditis for added diagnostic value of sonication to the standard microbiological workup in a prospective diagnostic proof of concept study. Two sonication protocols (broth enrichment vs. centrifugation) were compared in an additional 35 negative control valves for contamination rates. We selected sonication/centrifugation based on acceptable false positive rates (11.4%; 4/35). Sonication/enrichment yielded many false positive results in negative controls (28.6%; 10/35), mainly Propionibacterium acnes (next-generation sequencing excluded technical problems). Compared to direct culture only, adding sonication/centrifugation (including molecular testing) significantly increased the diagnostic yield from 6/26 to 17/26 valves (p = 0.003). Most importantly, culture positives almost doubled (from 6 to 10), providing unique quantitative information about antimicrobial susceptibility....Continue Reading

References

Aug 12, 1998·The Journal of Bone and Joint Surgery. British Volume·M M TunneyG Ramage
Apr 19, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J S LiG R Corey
Feb 13, 2001·Trends in Microbiology·T F Mah, G A O'Toole
Dec 26, 2001·Nucleic Acids Research·Dennis A BensonDavid L Wheeler
Jun 7, 2003·Biotechnology Progress·William G Pitt, S Aaron Ross
Oct 13, 2005·Journal of Long-term Effects of Medical Implants·Donald C Vinh, John M Embil
Aug 19, 2007·The New England Journal of Medicine·Andrej TrampuzRobin Patel
Mar 6, 2009·Journal of Clinical Microbiology·Kerryl E PiperRobin Patel
Oct 15, 2010·Journal of Clinical Microbiology·Alessandra OlivaClaudio M Mastroianni
Feb 22, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Johannes HolinkaElisabeth Presterl
Oct 11, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Fidel J VosWim J G Oyen
Feb 10, 2012·Lancet·Franck ThunyDidier Raoult
Dec 1, 2012·Journal of Clinical Microbiology·Alessandra OlivaClaudio M Mastroianni
Jul 2, 2014·Clinical Microbiology Reviews·Yvonne AchermannMark E Shirtliff
Sep 19, 2014·Journal of Clinical Microbiology·Maysa Harumi YanoMauro Jose Costa Salles
Nov 8, 2014·Pacing and Clinical Electrophysiology : PACE·Martin RohacekMaja Weisser
Feb 7, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K ZhouJ W A Rossen
Mar 18, 2015·The American Journal of Cardiology·Avish NagpalM Rizwan Sohail
Jul 29, 2015·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Ricardo Cantarim InacioMauro Jose Costa Salles
Nov 15, 2015·Advances in Experimental Medicine and Biology·Alessandra OlivaV Vullo
Jan 21, 2017·Journal of the American College of Cardiology·Thomas J CahillBernard D Prendergast

❮ Previous
Next ❯

Citations

Oct 17, 2019·Frontiers in Medicine·Manon KolbJacques Schrenzel
Jul 6, 2021·Frontiers in Cell and Developmental Biology·Christian Johann LercheClaus Moser

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJEB20613

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
CLC Genomics Workbench
SeqSphere +
cgMLST Target definer
SeqMan Pro
SeqSphere

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
Nelianne J VerkaikD C Melles
Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
M S SulkowskiD L Granger
© 2022 Meta ULC. All rights reserved