Sonoprinting and the importance of microbubble loading for the ultrasound mediated cellular delivery of nanoparticles

Biomaterials
I De CockI Lentacker

Abstract

In the last years, research on ultrasound mediated drug delivery using microbubbles is vastly expanding. While some groups simply mix drugs and microbubbles (co-administration), other researchers have a major interest in the potential of drug-loaded microbubbles. However, today, little is known on the pros and cons of these two strategies. In this study we evaluated the delivery of nanoparticles (polystyrene nanospheres and mRNA-lipoplexes) to cells in vitro, in case the nanoparticles were mixed with unloaded microbubbles versus loaded onto the microbubbles. Flow cytometry experiments demonstrated that unloaded microbubbles did not enhance the cellular delivery of the nanospheres and mRNA-lipoplexes. However, upon loading the nanoparticles onto the microbubbles, their delivery to cells substantially improved. Real-time swept field confocal microscopy imaging of the microbubbles and cells during ultrasound radiation revealed that nanoparticle-loaded microbubbles directly deposited the nanoparticles in patches onto the cell membrane, a process that we termed 'sonoprinting'. This phenomenon resulted in the delivery of large amounts of nanoparticles to the cells and is suggested to be different from the creation of cell membrane po...Continue Reading

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Citations

Nov 5, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Georg DimcevskiOdd Helge Gilja
Jun 8, 2017·Nanomaterials·Santhosh Kalash RajendrakumarIn-Kyu Park
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Oct 18, 2020·Pharmaceutics·Yoko Endo-Takahashi, Yoichi Negishi
Feb 8, 2021·Ultrasound in Medicine & Biology·Sofie SnipstadSigrid Berg
Jun 29, 2021·Advanced Drug Delivery Reviews·Sofie SnipstadCatharina de Lange Davies
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Jan 18, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Silke RooversIne Lentacker
Aug 13, 2018·ACS Biomaterials Science & Engineering·Yue LiZhi-Yi Chen
Aug 29, 2021·Pharmaceutics·Kristin Entzian, Achim Aigner

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