SopE acts as an Rab5-specific nucleotide exchange factor and recruits non-prenylated Rab5 on Salmonella-containing phagosomes to promote fusion with early endosomes

The Journal of Biological Chemistry
K MukherjeeA Mukhopadhyay

Abstract

Rab-GTPase regulates the fusion between two specific vesicles. It is well documented that, for their biological function, Rab proteins need to be prenylated for attachment to the vesicle membrane. In contrast, we showed in the present investigation that SopE, a type III secretory protein of Salmonella, translocates onto Salmonella-containing phagosomes (LSP) and mediates the recruitment of non-prenylated Rab5 (Rab5:DeltaC4) on LSP in GTP form. Simultaneously, SopE present in infected cell cytosol acts as an Rab5-specific exchange factor and converts the inactive Rab-GDP to the GTP form. The non-prenylated Rab5 subsequently promoted efficient fusion of LSP with early endosomes. This is the first demonstration that a prenylation-deficient Rab protein retains biological activity and can promote vesicle fusion, if it is recruited on the membrane by some other method.

References

Jan 1, 1992·International Review of Cytology·Y TakaiM Kawata
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K KatoC J Der
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R Khosravi-FarC J Der
Mar 8, 1991·Cell·J P GorvelJ Gruenberg
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·C C FarnsworthJ A Glomset
Dec 1, 1995·The Journal of Cell Biology·Y FengA Wandinger-Ness
Oct 1, 1995·Trends in Microbiology·F Garcia-del Portillo, B B Finlay
Mar 24, 1997·The Journal of Cell Biology·A MukhopadhyayP D Stahl
May 16, 1997·The Journal of Biological Chemistry·A MukhopadhyayP D Stahl
May 23, 1997·Science·J E Rothman, T H Söllner
Jun 27, 1997·The Journal of Biological Chemistry·K FunatoP D Stahl
Jun 2, 1998·Cellular Signalling·M C Seabra
Aug 26, 1998·The Journal of Biological Chemistry·F SchimmöllerS R Pfeffer
Aug 27, 1998·Electrophoresis·P Gromov, J E Celis
Jul 16, 1999·The EMBO Journal·K UchiyaE A Groisman
Dec 22, 1999·Trends in Microbiology·F García-Del Portillo
Jul 13, 2000·The Journal of Cell Biology·C Alory, W E Balch

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Citations

Mar 24, 2004·Current Opinion in Microbiology·John H Brumell, Sergio Grinstein
Jul 3, 2013·Experimental Cell Research·Konrad Krzewski, Andrew R Cullinane
Feb 14, 2002·Trends in Molecular Medicine·Miguel C SeabraAlistair N Hume
Nov 21, 2007·Nature Reviews. Microbiology·Andrea HaragaSamuel I Miller
Jan 16, 2010·Nature Reviews. Microbiology·Lautaro Diacovich, Jean-Pierre Gorvel
Apr 6, 2004·Molecular Biology of the Cell·Marije MarsmanJacques Neefjes
May 1, 2013·Infection and Immunity·Sushmita MustafiM Alejandro Barbieri
Feb 4, 2010·Infection and Immunity·Richard BulginAna Arbeloa
Feb 16, 2007·Journal of Virology·Michelle StoneKouacou V Konan
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·John H Brumell, Marci A Scidmore
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Joseph T BarbieriKlaus Aktories
Jan 31, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sébastien P FaucherFrance Daigle
Nov 30, 2011·The Journal of Cell Biology·Eric AlixCraig R Roy
Oct 14, 2014·International Journal of Molecular Sciences·Jun Hyuck LeeYong Ho Park
Feb 24, 2010·FEBS Letters·Seetharaman ParashuramanAmitabha Mukhopadhyay
Aug 21, 2012·Traffic·Mary-Pat SteinAngela Wandinger-Ness
Oct 10, 2014·Cell Host & Microbe·Nora MelloukJost Enninga
Nov 23, 2006·Microbes and Infection·Filip BoyenFreddy Haesebrouck
Aug 13, 2003·Traffic·Leigh A Knodler, Olivia Steele-Mortimer
Dec 20, 2007·Traffic·Carmen Alvarez-DominguezEugenio Carrasco-Marín
Jun 10, 2006·The EMBO Journal·Malavika BhattacharyaAmitabha Mukhopadhyay
Jan 4, 2012·Proteomics. Clinical Applications·Sylvia SchlekerJudith Klein-Seetharaman
May 15, 2002·International Journal of Medical Microbiology : IJMM·Michelle L ZaharikB Brett Finlay
Mar 4, 2014·Current Opinion in Microbiology·Elizabeth A Creasey, Ralph R Isberg
Jan 23, 2009·Current Opinion in Microbiology·Emma J McGhieVassilis Koronakis
Nov 2, 2016·Small GTPases·Noelia López-Montero, Jost Enninga
Jan 6, 2017·Small GTPases·Matthias P Müller, Roger S Goody
Mar 30, 2018·FEMS Microbiology Reviews·Eric MartinezMatteo Bonazzi
Jul 19, 2011·Nature Reviews. Microbiology·Hyeilin HamKim Orth
Feb 28, 2020·Molecular & Cellular Proteomics : MCP·Tatjana ReuterNicole Hansmeier
Dec 8, 2004·The Journal of Biological Chemistry·Ganga KrishnamurthyAmitabha Mukhopadhyay
Oct 20, 2001·The Journal of Biological Chemistry·A K CrissB A McCormick
Sep 26, 2020·Frontiers in Cellular and Infection Microbiology·Jiyao GanCristina Giogha
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Tatjana ReuterNicole Hansmeier
Jun 4, 2019·Microbiology Spectrum·Marcela De Souza Santos, Kim Orth

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