Sophorae radix extract inhibits high glucose-induced vascular cell adhesion molecule-1 up-regulation on endothelial cell line

Clinica Chimica Acta; International Journal of Clinical Chemistry
Kang-Beom KwonDo-Gon Ryu

Abstract

Sophorae radix (SR) has been used for various diseases including atherosclerosis and arrhythmias. Atherosclerosis induced by hyperglycemia is an important factor in the promotion of diabetic complications. An early event in atherosclerosis is the adhesion of monocytes to endothelium via adhesion molecules. Among them, vascular cell adhesion molecule-1 (VCAM-1) expression mediates the binding of monocytes and lymphocytes to vascular endothelial cells. The study was performed on vascular endothelial cells (ECV304 cells) that were pretreated with various concentrations of SR extract for 3 h before exposure with high glucose (55.5 mmol/l) for 48 h. The protein expression of VCAM-1 was measured by enzyme-linked immunosorbent assay (ELISA) and its mRNA expression was by reverse transcription polymerase chain reaction (RT-PCR). SR extract significantly inhibited high glucose-induced expression of VCAM-1 in a dose-dependent manner and reduced the level of VCAM-1 mRNA through interfering with translocation of nuclear factor-kappaB (NF-kappaB). Decreased VCAM-1 expression by SR extract was associated reduction of adherence between high glucose-stimulated ECV304 cells and human monocyte-like HL-60 cells. These data suggest that SR extract...Continue Reading

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Citations

Nov 26, 2010·Phytotherapy Research : PTR·K SpelmanE J Williams
Feb 3, 2015·Regulatory Toxicology and Pharmacology : RTP·Jeong-Hwan CheByeong-Cheol Kang
Apr 9, 2009·Journal of Pharmacological Sciences·Shrabanti DevHiroyuki Fukui
Jan 5, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroyuki Fukui

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