Sorption of amitriptyline and amphetamine to mixed-mode solid-phase microextraction in different test conditions

Journal of Chromatography. a
Hester PeltenburgIngrid J Bosman

Abstract

A solid-phase microextraction (SPME) method based on a sampler coating that includes strong cation groups (C18/SCX) is explored as a rapid direct sampling tool to detect and quantify freely dissolved basic drugs. Sampling kinetics, sorption isotherms and competitive effects on extraction yields in mixtures were tested for amphetamine and the relatively large/hydrophobic tricyclic antidepressant amitriptyline. Both compounds are >99% ionized at pH 7.4 but their affinity for the C18/SCX fiber is markedly different with distribution coefficients (Dfw values) of 2.49±0.02 for amphetamine and 4.72±0.10 for amitriptyline. Typical changes in electrolyte homeostasis that may occur in biomedical samples were simulated by altering pH and ionic composition (Na(+) and K(+) concentrations). These changes were shown to affect C18/SCX sorption affinities of the tested drugs with less than 0.2log units. At relatively low fiber loadings (<10mmol/L coating) and at all tested exposure times, linear sorption isotherms were obtained for both compounds but at aqueous concentrations of the individual drugs corresponding to concentrations in blood that are lethal, sorption isotherms became strongly nonlinear. Competition effects within binary mixtures...Continue Reading

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Citations

Aug 28, 2015·Journal of Pharmaceutical and Biomedical Analysis·Hester PeltenburgJoop L M Hermens
Apr 17, 2016·Journal of Chromatography. a·Hester PeltenburgSteven T J Droge
May 29, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kelly Zhang, Xiaodong Liu
Sep 21, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kelly Zhang, Xiaodong Liu

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