Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae.

Autophagy
Yi Zhen DengNaweed I Naqvi

Abstract

The sorting nexins Atg20/Snx42 and Snx41 regulate membrane traffic and endosomal protein sorting and are essential for Cvt and/or pexophagy in yeast. Previously, we showed that macroautophagy is necessary for conidiation in the rice-blast fungus Magnaporthe oryzae. Here, we analyzed the physiological function(s) of selective autophagy in Magnaporthe through targeted deletion of MGG_12832, an ortholog of yeast SNX41 and ATG20/SNX42. Loss of MGG_12832 (hereafter SNX41) abolished conidia formation and pathogenesis in M. oryzae. Snx41-GFP localized as dynamic puncta or short tubules that are partially associated with autophagosomes and/or autophagic vacuoles. PX domain, but not macroautophagy per se, was required for such localization of Snx41-GFP in Magnaporthe. Although not required for nonselective autophagy, Snx41 was essential for pexophagy in Magnaporthe. We identified Oxp1, an ATP-dependent oxoprolinase in the gamma-glutamyl cycle, as a binding partner and potential retrieval target of Snx41-dependent protein sorting. The substrate of Oxp1, 5-oxoproline, could partially restore conidiation in the snx41Δ. Exogenous glutathione, a product of the gamma-glutamyl cycle, significantly restored pathogenicity in the snx41Δ mutant, l...Continue Reading

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Citations

Dec 5, 2013·PloS One·Yizhen DengNaweed I Naqvi
Aug 11, 2015·Nature Chemical Biology·Rajesh N PatkarNaweed I Naqvi
Dec 7, 2017·Frontiers in Microbiology·Poonguzhali SelvarajNaweed I Naqvi
Nov 2, 2016·Journal of Cell Science·Dan ZhaoLi-Lin Du
Oct 23, 2018·Environmental Microbiology·Qinhu WangJin-Rong Xu
Nov 27, 2018·Virulence·Xue-Ming ZhuFu-Cheng Lin
May 6, 2020·The New Phytologist·Qing ShenNaweed I Naqvi
Jul 10, 2019·Annual Review of Microbiology·Yi Zhen Deng, Naweed I Naqvi
Jun 16, 2017·PLoS Pathogens·Rajesh N Patkar, Naweed I Naqvi

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