Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway.

RNA
Megha GhildiyalP D Zamore

Abstract

In flies, small silencing RNAs are sorted between Argonaute1 (Ago1), the central protein component of the microRNA (miRNA) pathway, and Argonaute2 (Ago2), which mediates RNA interference. Extensive double-stranded character-as is found in small interfering RNAs (siRNAs)-directs duplexes into Ago2, whereas central mismatches, like those found in miRNA/miRNA* duplexes, direct duplexes into Ago1. Central to this sorting decision is the affinity of the small RNA duplex for the Dcr-2/R2D2 heterodimer, which loads small RNAs into Ago2. Here, we show that while most Drosophila miRNAs are bound to Ago1, miRNA* strands accumulate bound to Ago2. Like siRNA loading, efficient loading of miRNA* strands in Ago2 favors duplexes with a paired central region and requires both Dcr-2 and R2D2. Those miRNA and miRNA* sequences bound to Ago2, like siRNAs diced in vivo from long double-stranded RNA, typically begin with cytidine, whereas Ago1-bound miRNA and miRNA* disproportionately begin with uridine. Consequently, some pre-miRNA generate two or more isoforms from the same side of the stem that differentially partition between Ago1 and Ago2. Our findings provide the first genome-wide test for the idea that Drosophila small RNAs are sorted between...Continue Reading

References

Jan 5, 2000·Genes & Development·T TuschlP A Sharp
Oct 27, 2001·Science·M Lagos-QuintanaT Tuschl
Oct 27, 2001·Science·R C Lee, V Ambros
May 20, 2003·Nature Biotechnology·Aimee L JacksonPeter S Linsley
Jun 28, 2003·Methods : a Companion to Methods in Enzymology·Young Sik Lee, Richard W Carthew
Sep 26, 2003·Nature·Yoontae LeeV Narry Kim
Oct 22, 2003·Cell·Dianne S SchwarzPhillip D Zamore
Oct 22, 2003·Cell·Anastasia KhvorovaSumedha D Jayasena
Nov 25, 2003·Science·Elsebet LundUlrike Kutay
Dec 19, 2003·Genes & Development·Rui YiBryan R Cullen
Dec 31, 2003·Cell·Benjamin P LewisChristopher B Burge
Mar 12, 2004·Developmental Biology·Nikolaus Rajewsky, Nicholas D Socci
Mar 24, 2004·Cell·Yukihide TomariPhillip D Zamore
Jun 1, 2004·Nature Structural & Molecular Biology·Benjamin Haley, Phillip D Zamore
Jul 3, 2004·Genes & Development·Katsutomo OkamuraMikiko C Siomi
Sep 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tim A RandXiaodong Wang
Nov 9, 2004·Nature·Richard I GregoryRamin Shiekhattar
Nov 9, 2004·Nature·Ahmet M DenliGregory J Hannon
Nov 20, 2004·Science·Yukihide TomariPhillip D Zamore
Dec 3, 2004·Genes & Development·Jinju HanV Narry Kim
Apr 5, 2005·Nature Genetics·Azra KrekNikolaus Rajewsky
May 28, 2005·PLoS Biology·Kuniaki SaitoMikiko C Siomi
Jun 24, 2005·Nature·Thimmaiah P ChendrimadaRamin Shiekhattar
Jun 30, 2005·Genes & Development·Feng JiangQinghua Liu
Aug 17, 2005·PLoS Computational Biology·Dominic GrünNikolaus Rajewsky
Nov 17, 2005·Genes & Development·Keita MiyoshiMikiko C Siomi
Dec 17, 2005·Genes & Development·Elisavet Maniataki, Zissimos Mourelatos
Jan 21, 2006·The EMBO Journal·Yoontae LeeV Narry Kim
Jan 28, 2006·EMBO Reports·Philipp J F LeuschnerJavier Martinez

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Citations

Jul 25, 2014·BMC Genomics·Ammar NaqviAndrey Grigoriev
Jan 1, 2014·Journal of Bioinformatics and Computational Biology·Igor I Titov, Pavel S Vorozheykin
Mar 15, 2014·Nucleic Acids Research·Natsuko YamakawaAi Kotani
Jan 29, 2014·Cellular and Molecular Life Sciences : CMLS·Anna Stroynowska-CzerwinskaWlodzimierz J Krzyzosiak
Apr 20, 2014·The Journal of Clinical Investigation·Claudia BangThomas Thum
Apr 18, 2014·Neurosurgery·Antonio Di IevaMichael D Cusimano
Aug 5, 2014·Annual Review of Phytopathology·Arne WeibergHailing Jin
Jul 4, 2012·Annual Review of Microbiology·Shwu-Shin ChangYi Liu
Jul 17, 2014·Nature Reviews. Molecular Cell Biology·Minju Ha, V Narry Kim
May 10, 2011·Annual Review of Nutrition·Kathryn J MooreCarlos Fernández-Hernando
Nov 20, 2014·Nature Communications·Xiaoming ZhangHailing Jin
Feb 27, 2010·Biochemistry·Joseph A GredellS Patrick Walton
Sep 2, 2010·RNA·Selene L Fernandez-ValverdeJohn S Mattick
Oct 29, 2010·Journal of Virology·Christine MeyerDaniel N Streblow
Dec 1, 2010·Nature Reviews. Genetics·Benjamin Czech, Gregory J Hannon
Dec 24, 2010·RNA·Jr-Shiuan YangEric C Lai
May 7, 2011·Nucleic Acids Research·Zheng YanPhilipp Khaitovich
May 11, 2011·Genome Biology·Michael J AxtellEric C Lai
May 25, 2011·Cellular and Molecular Life Sciences : CMLS·Julia Starega-RoslanWlodzimierz J Krzyzosiak
Jan 3, 2012·Genome Biology·Nicole CloonanSean M Grimmond
Mar 1, 2012·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Yu FangJianguo Hu
Sep 29, 2012·Silence·Antonio MarcoSam Griffiths-Jones
Feb 5, 2013·Nucleic Acids Research·Ying Poi LiuBen Berkhout
Sep 7, 2013·PLoS Pathogens·Joao Trindade MarquesRichard W Carthew

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