PMID: 9523707Apr 2, 1998Paper

Sorting of invertase signal peptide mutants in yeast dependent and independent on the signal-recognition particle

European Journal of Biochemistry
C Rothe, L Lehle

Abstract

There is growing evidence that yeast contains two efficient pathways of protein translocation across the endoplasmic reticulum membrane, one dependent on the signal-recognition particle (SRP) and the other independent. Their specificity, however, is largely obscure. For higher eukaryotes it has been shown that a high average hydrophobicity of the core region with a minimal length around six or seven amino acids, as well as a stabilized alpha-helix, are decisive structural features for translocation. Using yeast invertase as a secretory model protein, we have found that mutated signal sequences with Pro or Gly in the core, or having only four hydrophobic amino acids, are not functional in translocation across microsomal membranes of dog pancreas because they do not interact with the SRP. Expression of these mutant variants in Saccharomyces cerevisiae revealed that they are sorted independently of the SRP since translocation was not impaired in an SRP-deficient yeast strain. In contrast to this, wild-type invertase is translocated SRP-dependently in wild-type cells and shows a decreased translocation in SRP-deficient cells. By overexpression of Srp54p, but not of Hsc70p, the translocation defect of wild-type invertase in an SRP54...Continue Reading

Citations

Sep 28, 2000·Parasitology Today·G G van DoorenG I McFadden
Dec 23, 1999·Current Opinion in Structural Biology·R M Stroud, P Walter
Nov 25, 2004·European Journal of Biochemistry·Toshihiro YasudaKoichiro Kishi
Jun 8, 2001·Annual Review of Biochemistry·R J KeenanP Walter
Dec 27, 2005·Bioscience, Biotechnology, and Biochemistry·Cenk KigGüler Temizkan
Apr 5, 2011·Protoplasma·Verena KriechbaumerBen M Abell
Jan 18, 2006·Letters in Applied Microbiology·S TürkelN Kayakent
Mar 28, 2013·Critical Reviews in Biochemistry and Molecular Biology·Tslil Ast, Maya Schuldiner
Mar 20, 1999·The Journal of Biological Chemistry·S Strahl-Bolsinger, A Scheinost
Mar 26, 2003·Molecular Genetics and Genomics : MGG·S TürkelH V Baker
Apr 19, 2003·The Journal of Biological Chemistry·Daniel BehaHans-Georg Koch
Jun 7, 2017·Cytotechnology·Yuki Ohmuro-Matsuyama, Hideki Yamaji

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