PMID: 9524075Apr 2, 1998Paper

Sorting of non-glycosylated human procathepsin S in mammalian cells

Biological Chemistry
K NisslerB Wiederanders

Abstract

Cathepsin S, a lysosomal cysteine protease, is synthesized as inactive precursor. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. HEK 293-cells which do not express cathepsin S were transfected with cDNA of either wild type human procathepsin S or a mutant procathepsin S in which Asn of the only glycosylation site in the proregion was replaced by Gln. The cells expressed glycosylated and non-glycosylated procathepsin S, respectively. Large amounts of the precursors were secreted into the culture media by both transfectants. Secreted wild type procathepsin S contained Man-6-phosphate in the oligosaccharide chain. Wild type procathepsin S was activated in the cells but no maturation occurred in the culture media. In vitro processing of glycosylated as well as of non-glycosylated procathepsin S gave fully active enzymes thus indicating that the oligosaccharide chain was not necessary for proper folding. A reuptake of the glycosylated and non-glycosylated procathepsin S by HEK 293-cells could be observed. Small amounts of mature cathepsin S were detected in the lysosomes of the mutant transfectants. Subcellular fractionation showed non-glycosylated procathepsin S in the membrane fraction. Non-glycosyla...Continue Reading

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Citations

Feb 22, 2001·Biochemical and Biophysical Research Communications·D Claveau, D Riendeau
Oct 26, 2011·Biochimica Et Biophysica Acta·Vito TurkDušan Turk
Jun 9, 2009·The Journal of Allergy and Clinical Immunology·Victoria M LebWinfried F Pickl
Nov 27, 2007·The Journal of Allergy and Clinical Immunology·Victoria M LebWinfried F Pickl
Jun 23, 2009·European Journal of Immunology·Judith LeitnerPeter Steinberger
Sep 1, 1999·European Journal of Biochemistry·K NisslerB Wiederanders
Apr 25, 2007·British Journal of Neurosurgery·T FlanneryP G Johnston
Apr 14, 2017·Oncotarget·Siyuan ChenHong Guo
Jan 30, 2003·European Journal of Cell Biology·Virginie SantilmanFrancis Mainferme
Dec 30, 2021·Biomedit︠s︡inskai︠a︡ khimii︠a︡·T A GureevaN I Solovyova

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