PMID: 376772Jan 1, 1979Paper

Sorting out behaviour of disaggregated cells in the absence of morphogenesis in Dictyostelium discoideum

Journal of Embryology and Experimental Morphology
M Tasaka, I Takeuchi

Abstract

Cells disaggregated from slugs of Dictyostelium discoideum were cultured in Bonner's salt solution in roller tubes. Cells rapidly stuck together to form an amorphous loose agglutinate which was later transformed into a spheroidal tight agglutinate surrounded by slime sheath material. Prespore cells in the loose agglutinate underwent partial dedifferentiation by starting to decompose their specific antigen until formation of the tight agglutinate, in which the antigen was resynthesized. During the process, there was some decrease in the proportion of prespore cells. Changes in the distribution of prespore and prestalk cells in the agglutinates were examined by using immunocytochemical staining. They were randomly distributed in the early agglutinates, but became well separated in 4 h agglutinates in such a way that prestalk cells were completely enveloped by prespore cells. Prestalk cells later came outside to be partially enveloped and finally occupied a hemisphere side by side with prespore cells. During the process, cells in one or two outer layers differentiated into prestalk or stalk cells. Similar changes in the distribution pattern were observed, when labelled prestalk cells were cultured with unlabelled prespore cells an...Continue Reading

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