Sorting out mix-ups. The provenance of tissue sections may be confirmed by PCR using microsatellite markers

American Journal of Clinical Pathology
D S O'BriainM Lawler

Abstract

Standard identification systems usually ensure that biopsy material is correctly associated with a given patient. Sometimes, as when a tumor is unexpectedly found, the provenance (proof of origin) of a tissue sample may be questioned; the tissue may have been mislabelled or contaminated with tissue from another patient. Techniques used to confirm tissue provenance include comparing either tissue markers of gender or ABO blood groups; however, these methods have weak confirmatory power. Recently, the use of DNA-based polymerase chain reaction (PCR) techniques has been reported. Paired, formalin-fixed, paraffin-embedded, 10 microns tissue sections were selected from 17 patients, 8 of whom had carcinoma, either by dividing a biopsy section, using sequential biopsies, or sequential biopsy and autopsy tissue. The resulting 36 samples were coded before analysis. In two additional cases, 1-mm fragments of tumor from one patient were included in the tissue block of benign tissue from another patient, the tumor fragments were identified on hematoxylin-and-eosin-stained sections, separately scraped off the glass slide, and analyzed. Tissue from two clinical cases, one of suspected mislabelling and one with a suspected carry-over of malig...Continue Reading

Citations

Jun 22, 2007·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Valérie DubéTerence J Colgan
Jun 27, 2008·Advances in Anatomic Pathology·Jennifer L Hunt
May 28, 2011·Journal of Clinical Pathology·Sepideh AminTim C Diss
Sep 18, 2012·The American Journal of Surgical Pathology·Sarah ChiangA John Iafrate
Mar 16, 2005·The American Journal of Surgical Pathology·Christopher A Moskaluk
Oct 28, 1999·Journal of Healthcare Risk Management : the Journal of the American Society for Healthcare Risk Management·R E Wenk
Dec 8, 2004·Archives of Pathology & Laboratory Medicine·Jennifer L Hunt, Sydney D Finkelstein
Feb 4, 2003·Archives of Pathology & Laboratory Medicine·Jennifer L HuntSydney D Finkelstein

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