SOS response in bacteria: Inhibitory activity of lichen secondary metabolites against Escherichia coli RecA protein

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Pierangelo BellioGiuseppe Celenza

Abstract

RecA is a bacterial multifunctional protein essential to genetic recombination, error-prone replicative bypass of DNA damages and regulation of SOS response. The activation of bacterial SOS response is directly related to the development of intrinsic and/or acquired resistance to antimicrobials. Although recent studies directed towards RecA inactivation via ATP binding inhibition described a variety of micromolar affinity ligands, inhibitors of the DNA binding site are still unknown. Twenty-seven secondary metabolites classified as anthraquinones, depsides, depsidones, dibenzofurans, diphenyl-butenolides, paraconic acids, pseudo-depsidones, triterpenes and xanthones, were investigated for their ability to inhibit RecA from Escherichia coli. They were isolated in various Chilean regions from 14 families and 19 genera of lichens. The ATP hydrolytic activity of RecA was quantified detecting the generation of free phosphate in solution. The percentage of inhibition was calculated fixing at 100µM the concentration of the compounds. Deeper investigations were reserved to those compounds showing an inhibition higher than 80%. To clarify the mechanism of inhibition, the semi-log plot of the percentage of inhibition vs. ATP and vs. ssDN...Continue Reading

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Citations

Oct 31, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alfredo Torres-BenítezCarlos Areche
Feb 13, 2020·Natural Product Reports·Marjorie BruderDaniela B B Trivella
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Nov 11, 2020·Applied Microbiology and Biotechnology·Heesu Kim, Dong Gun Lee
Feb 9, 2021·Computational and Structural Biotechnology Journal·Alexander YakimovDmitry Baitin
May 26, 2021·Planta medica·Isabel Ureña-VacasM Pilar Gómez-Serranillos

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