SOS System Induction Inhibits the Assembly of Chemoreceptor Signaling Clusters in Salmonella enterica

PloS One
Oihane IrazokiJ Barbé

Abstract

Swarming, a flagellar-driven multicellular form of motility, is associated with bacterial virulence and increased antibiotic resistance. In this work we demonstrate that activation of the SOS response reversibly inhibits swarming motility by preventing the assembly of chemoreceptor-signaling polar arrays. We also show that an increase in the concentration of the RecA protein, generated by SOS system activation, rather than another function of this genetic network impairs chemoreceptor polar cluster formation. Our data provide evidence that the molecular balance between RecA and CheW proteins is crucial to allow polar cluster formation in Salmonella enterica cells. Thus, activation of the SOS response by the presence of a DNA-injuring compound increases the RecA concentration, thereby disturbing the equilibrium between RecA and CheW and resulting in the cessation of swarming. Nevertheless, when the DNA-damage decreases and the SOS response is no longer activated, basal RecA levels and thus polar cluster assembly are reestablished. These results clearly show that bacterial populations moving over surfaces make use of specific mechanisms to avoid contact with DNA-damaging compounds.

References

Feb 1, 1992·Journal of Medical Microbiology·E G Power, I Phillips
Mar 5, 1990·Journal of Molecular Biology·M Sassanfar, J W Roberts
Jul 1, 1990·Molecular Microbiology·L McCarter, M Silverman
Nov 1, 1987·Journal of Bacteriology·B SallesG M Weinstock
Oct 1, 1985·Environmental Health Perspectives·G C WalkerL Dodson
Jan 1, 1982·Molecular & General Genetics : MGG·H GinsburgD W Mount
May 1, 1982·Cell·J W Little, D W Mount
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·R M Harshey, T Matsuyama
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·C CayrolM Defais
Nov 1, 1996·Journal of Bacteriology·O RauprichJ A Shapiro
Jun 1, 1997·Molecular Microbiology·K M Ottemann, J F Miller
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M BurkartR M Harshey
Oct 3, 1999·Progress in Nucleic Acid Research and Molecular Biology·M M Cox
Apr 12, 2000·Molecular Microbiology·A R Fernández De HenestrosaR Woodgate
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jul 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michal RonenUri Alon
Oct 7, 2003·Annual Review of Microbiology·Rasika M Harshey
Jun 4, 2004·Journal of Bacteriology·Mingshan Li, Gerald L Hazelbauer
Oct 20, 2004·Molecular Microbiology·Wook Kim, Michael G Surette
Dec 22, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Melinda D BakerJeffry B Stock
Jun 27, 2006·Molecular Microbiology·Susana MaricondaRasika M Harshey
Jul 22, 2006·Molecular Microbiology·David KentnerVictor Sourjik
Mar 30, 2007·BMC Biology·José-María Gómez-GómezJesús Blázquez
Sep 22, 2007·FEMS Microbiology Reviews·Ivan ErillJordi Barbé
May 23, 2009·Science·Emilie GuerinDidier Mazel
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ariane BriegelGrant J Jensen
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mitchell T ButlerRasika M Harshey
Feb 18, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shannon E MajowiczUNKNOWN International Collaboration on Enteric Disease 'Burden of Illness' Studies

❮ Previous
Next ❯

Citations

Feb 6, 2020·Frontiers in Microbiology·Alejandra AlvaradoChristine Josenhans
Oct 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Brandon H SchlomannRaghuveer Parthasarathy
Apr 23, 2020·Frontiers in Microbiology·Elisabet Frutos-GriloSusana Campoy

❮ Previous
Next ❯

Methods Mentioned

BETA
co-immunoprecipitation
PCR
ELISA
fluorescence microscopy

Software Mentioned

ImageJ
Prism ( GraphPad )

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.