SOT1, a pentatricopeptide repeat protein with a small MutS-related domain, is required for correct processing of plastid 23S-4.5S rRNA precursors in Arabidopsis thaliana

The Plant Journal : for Cell and Molecular Biology
Wenjuan WuJirong Huang

Abstract

Ribosomal RNA processing is essential for plastid ribosome biogenesis, but is still poorly understood in higher plants. Here, we show that SUPPRESSOR OF THYLAKOID FORMATION1 (SOT1), a plastid-localized pentatricopeptide repeat (PPR) protein with a small MutS-related domain, is required for maturation of the 23S-4.5S rRNA dicistron. Loss of SOT1 function leads to slower chloroplast development, suppression of leaf variegation, and abnormal 23S and 4.5S processing. Predictions based on the PPR motif sequences identified the 5' end of the 23S-4.5S rRNA dicistronic precursor as a putative SOT1 binding site. This was confirmed by electrophoretic mobility shift assay, and by loss of the abundant small RNA 'footprint' associated with this site in sot1 mutants. We found that more than half of the 23S-4.5S rRNA dicistrons in sot1 mutants contain eroded and/or unprocessed 5' and 3' ends, and that the endonucleolytic cleavage product normally released from the 5' end of the precursor is absent in a sot1 null mutant. We postulate that SOT1 binding protects the 5' extremity of the 23S-4.5S rRNA dicistron from exonucleolytic attack, and favours formation of the RNA structure that allows endonucleolytic processing of its 5' and 3' ends.

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Citations

Dec 9, 2015·The Plant Journal : for Cell and Molecular Biology·Reimo ZoschkeAlice Barkan
Mar 19, 2016·Biochimica Et Biophysica Acta·Joanna MelonekKarin Krupinska
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Feb 9, 2018·The Plant Journal : for Cell and Molecular Biology·Julia LegenChristian Schmitz-Linneweber
Nov 6, 2018·The New Phytologist·Hannes RuwePeter Kindgren
Apr 20, 2019·The Plant Journal : for Cell and Molecular Biology·Giada MarinoDario Leister
Aug 24, 2019·The Plant Journal : for Cell and Molecular Biology·Kwanuk LeeHunseung Kang
Feb 9, 2017·Proceedings of the National Academy of Sciences of the United States of America·Wen ZhouCongming Lu
Jul 2, 2020·International Journal of Molecular Sciences·Kwanuk Lee, Hunseung Kang
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Dec 29, 2020·The Plant Journal : for Cell and Molecular Biology·Tatjana KleineDario Leister
Apr 3, 2020·Cell Reports·Marissa L GloverJoshua A Arribere

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