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Sources of PCR-induced distortions in high-throughput sequencing datasets

bioRxiv

Aug 23, 2014

Justus M. Kebschull, Anthony M. Zador

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Abstract

PCR permits the exponential and sequence-specific amplification of DNA, even from minute starting quantities. PCR is a fundamental step in preparing DNA samples for high-throughput sequencing. However, there are errors associated with PCR-mediated amplification. Here we examine the effe...read more

Mentioned in this Paper

Polymerase Chain Reaction
Genome Sequencing
DNA Amplification
cDNA Library
High-Throughput RNA Sequencing
Structure
Polymerase
Abnormal Shape
Gene Amplification Technique
High Throughput Analysis
41
11
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  • Sources of PCR-induced distortions in high-throughput sequencing datasets

    bioRxiv

    Aug 23, 2014

    Justus M. Kebschull, Anthony M. Zador

    PMID: 990008375

    DOI: 10.1101/008375

    Abstract

    PCR permits the exponential and sequence-specific amplification of DNA, even from minute starting quantities. PCR is a fundamental step in preparing DNA samples for high-throughput sequencing. However, there are errors associated with PCR-mediated amplification. Here we examine the effe...read more

    Mentioned in this Paper

    Polymerase Chain Reaction
    Genome Sequencing
    DNA Amplification
    cDNA Library
    High-Throughput RNA Sequencing
    41
    11

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