Sources of variation in oxygen consumption of aquatic animals demonstrated by simulated constant oxygen consumption and respirometers of different sizes

Journal of Fish Biology
M B S SvendsenJ F Steffensen

Abstract

As intermittent-flow respirometry has become a common method for the determination of resting metabolism or standard metabolic rate (SMR), this study investigated how much of the variability seen in the experiments was due to measurement error. Experiments simulated different constant oxygen consumption rates (M˙O2 ) of a fish, by continuously injecting anoxic water into a respirometer, altering the injection rate to correct for the washout error. The effect of respirometer-to-fish volume ratio (RFR) on SMR measurement and variability was also investigated, using the simulated constant M˙O2 and the M˙O2 of seven roach Rutilus rutilus in respirometers of two different sizes. The results show that higher RFR increases measurement variability but does not change the mean SMR established using a double Gaussian fit. Further, the study demonstrates that the variation observed when determining oxygen consumption rates of fishes in systems with reasonable RFRs mainly comes from the animal, not from the measuring equipment.

References

Dec 6, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Mauricio A UrbinaMalcolm E Forster
Jan 1, 1989·Fish Physiology and Biochemistry·J F Steffensen
Jan 16, 2016·Journal of Fish Biology·D ChabotA P Farrell

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Citations

Nov 21, 2015·Journal of Fish Biology·T Norin, T D Clark
Nov 19, 2015·Journal of Fish Biology·E J Eliason, A P Farrell
Jan 16, 2016·Journal of Fish Biology·D ChabotA P Farrell
Sep 12, 2015·Journal of Fish Biology·S LefevreD J McKenzie
Jan 16, 2016·Journal of Fish Biology·D ChabotA P Farrell
Apr 26, 2016·ZooKeys·Cárlison Silva-OliveiraFrank Raynner Vasconcelos Ribeiro
Sep 7, 2016·Global Change Biology·Adam HabaryJodie L Rummer
Nov 28, 2017·PloS One·Richard S A WhiteAngus R McIntosh
Sep 23, 2016·The Journal of Experimental Biology·Lauren E NadlerMark I McCormick
May 6, 2019·The Journal of Experimental Biology·Yangfan ZhangAnthony P Farrell
Feb 13, 2019·Conservation Physiology·Sergey MorozovJuha Merilä
Nov 15, 2019·The Journal of Experimental Biology·Gordon R Ultsch, Matthew D Regan
Jun 15, 2018·Frontiers in Physiology·Mingzhe YuanWeiqun Lu
Sep 13, 2019·The Journal of Experimental Biology·Jessica E Reemeyer, Bernard B Rees
Feb 26, 2021·The Journal of Experimental Biology·Sjannie LefevreDavid J McKenzie
Aug 20, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Sonia A Aranda-MoralesCarlos A Álvarez-González
Sep 15, 2021·The Journal of Experimental Biology·Shaun S KillenDavid J McKenzie

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