PMID: 8588747Dec 1, 1995Paper

Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

Archives of Microbiology
M FujitaK Amako

Abstract

Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.

References

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Jun 1, 1995·Journal of Clinical Microbiology·M FujitaK Amako
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M K Tummuru, M J Blaser

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