Sox11 is expressed in early progenitor human multipotent stromal cells and decreases with extensive expansion of the cells.

Tissue Engineering. Part a
Benjamin L LarsonDarwin J Prockop

Abstract

There has been considerable interest in developing new therapies with adult multipotent progenitor stromal cells or mesenchymal stem cells (MSCs) in organ replacement and repair. To be effectively seeded into scaffolds for therapy, large numbers of cells are needed, but concerns remain regarding their chromatin stability in long-term culture. We therefore expanded four donors of human MSCs (hMSCs) from bone marrow aspirates with a protocol that maintains the cells at low density. MSCs initially proliferated at average doubling times of 24  h and then gradually reached senescence after 8-15 passages (33-55 population doublings) without evidence of immortalization. Comparative genomic hybridization assays of two preparations revealed no abnormalities through 33 population doublings. One preparation had a small amplification of unknown significance in chromosome 7 (7q21:11) after 55 population doublings. Microarray assays demonstrated progressive changes in the transcriptome of the cells. However, the transcriptomes clustered more closely over time within a single passage, rather than with passage number, indicating a partial reversibility of the patterns of gene expression. One of the largest changes was a decrease in mRNA for So...Continue Reading

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Citations

Sep 21, 2011·Cell Research·Rene RodriguezPablo Menendez
Mar 20, 2013·Breast Cancer Research : BCR·Marketa ZvelebilBeatrice A Howard
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Apr 9, 2021·PLoS Genetics·Keira J A JohnstonMark E S Bailey

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Methods Mentioned

BETA
chips
transfection
light microscopy
flow cytometry

Software Mentioned

dChip
GCOS
GeneChip Operating software
Partek Genomics Solution

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