Sox21 Regulates Anapc10 Expression and Determines the Fate of Ectodermal Organ

IScience
Kan SaitoSatoshi Fukumoto

Abstract

The transcription factor Sox21 is expressed in the epithelium of developing teeth. The present study aimed to determine the role of Sox21 in tooth development. We found that disruption of Sox21 caused severe enamel hypoplasia, regional osteoporosis, and ectopic hair formation in the gingiva in Sox21 knockout incisors. Differentiation markers were lost in ameloblasts, which formed hair follicles expressing hair keratins. Molecular analysis and chromatin immunoprecipitation sequencing indicated that Sox21 regulated Anapc10, which recognizes substrates for ubiquitination-mediated degradation, and determined dental-epithelial versus hair follicle cell fate. Disruption of either Sox21 or Anapc10 induced Smad3 expression, accelerated TGF-β1-induced promotion of epithelial-to-mesenchymal transition (EMT), and resulted in E-cadherin degradation via Skp2. We conclude that Sox21 disruption in the dental epithelium leads to the formation of a unique microenvironment promoting hair formation and that Sox21 controls dental epithelial differentiation and enamel formation by inhibiting EMT via Anapc10.

References

Dec 1, 1970·Annals of Internal Medicine·P D Saville
Jan 26, 1996·Biochemical and Biophysical Research Communications·S IsekiS Noji
Oct 13, 1998·Current Biology : CB·B St-JacquesA P McMahon
Oct 4, 2000·Nature Genetics·J LamartineG Waksman
Jun 15, 2001·The Journal of Biological Chemistry·C W GibsonA B Kulkarni
Mar 21, 2002·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Kazuo KomiyamaItaru Moro
May 22, 2002·International Review of Cytology·Irma Thesleff, Marja Mikkola
Oct 29, 2002·Nature Cell Biology·Christopher W Carroll, David O Morgan
Feb 7, 2003·American Journal of Human Genetics·Victor L Ruiz-PerezJudith A Goodship
Nov 25, 2003·Pathology International·Yasunori TakedaShin-ichi Nakamura
Feb 20, 2004·Human Mutation·Antonio PizzutiBruno Dallapiccola
Dec 8, 2004·The Journal of Cell Biology·Satoshi FukumotoYoshihiko Yamada
Dec 25, 2004·Differentiation; Research in Biological Diversity·Michael A RogersJürgen Schweizer
Jul 5, 2005·Nature Neuroscience·Magnus SandbergJonas Muhr
Dec 5, 2006·Developmental Biology·Amy L DonnerRichard L Maas
Nov 30, 2007·The Journal of Biological Chemistry·Keigo YoshizakiSatoshi Fukumoto
Jan 31, 2008·European Journal of Human Genetics : EJHG·Annemarie H van der HoutAnthonie J van Essen
Oct 29, 2008·The International Journal of Developmental Biology·Doreen D CunninghamElena Silva Casey
Apr 14, 2009·American Journal of Medical Genetics. Part a·Maria KirchhoffMargarita Stefanova
May 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Makoto KisoYumiko Saga
Jul 10, 2009·The Journal of Investigative Dermatology·Lutz LangbeinJuergen Schweizer
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Feb 1, 2012·PloS One·Ross A PochéMary E Dickinson
Feb 3, 2012·The Journal of Biological Chemistry·Makiko ArakakiSatoshi Fukumoto
Jul 10, 2012·Cell·Hiroyuki InuzukaWenyi Wei

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Citations

Dec 2, 2020·International Journal of Molecular Sciences·Keigo YoshizakiYuko Oda
Apr 13, 2021·Journal of Cellular Physiology·Shahad Al ThaminSatoshi Fukumoto

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Datasets Mentioned

BETA
GSE99359
GSE99360

Methods Mentioned

BETA
X-ray
immunoprecipitation
ubiquitination
transfection
fluorescence microscopy
Fluorescence

Software Mentioned

JASPAR
ImageJ
IGV

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