SOX9 and myocardin counteract each other in regulating vascular smooth muscle cell differentiation.

Biochemical and Biophysical Research Communications
Zhonghui XuLi Li

Abstract

Transdifferentiation of vascular smooth muscle cells (VSMC) into chondrogenic cells contributes significantly to vascular calcification during the pathogenesis of atherosclerosis. However, the transcriptional mechanisms that control such phenotypic switch remain unclear. This process is characterized by the induction of Sox9 and Col2a1 genes accompanied by the repression of myocardin (Myocd) and SMC differentiation markers such as SM22, SM α-actin and SM-MHC. Here we explore the regulatory role of SOX9, the master regulator for chondrogenesis, in modulating SMC marker gene expression. qRT-PCR and luciferase assays show that over-expression of SOX9 inhibits SMC gene transcription and promoter activities induced by myocardin, the master regulator of smooth muscle differentiation. Such suppression is independent of the CArG box in the SMC promoters but dependent on myocardin. EMSA assay further shows that SOX9 neither participates in SRF (serum response factor) binding to the CArG box nor interacts with SRF, while co-immunoprecipitation demonstrates an association of SOX9 with myocardin. Conversely, myocardin suppresses SOX9-mediated chondrogenic gene Col2a1 expression. These findings provide the first mechanistic insights into th...Continue Reading

References

Oct 24, 2002·Journal of Molecular and Cellular Cardiology·Jiyuan ChenJoseph M Miano
Jun 6, 2003·Journal of Molecular and Cellular Cardiology·Joseph M Miano
Oct 29, 2003·Circulation·Yin TintutLinda L Demer
Jan 31, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Frédéric Dandré, Gary K Owens
Jul 23, 2004·Physiological Reviews·Gary K OwensBrian R Wamhoff
Dec 30, 2004·The Journal of Biological Chemistry·Yan LiuGary K Owens
Aug 22, 2006·Journal of Internal Medicine·V P IyemereC M Shanahan
Oct 13, 2006·Molecular and Cellular Biology·Ken'ichiro HayashiKenji Sobue
Nov 30, 2007·Journal of Cellular Physiology·Haibing PengMary B Goldring
Jun 1, 2010·The Journal of Biological Chemistry·Jiliang ZhouXiaobo Wang
Dec 25, 2010·Cardiovascular Research·Jianbin ShenLi Li

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Citations

Jun 19, 2016·Biochimica Et Biophysica Acta·Enikő BaloghViktória Jeney
Oct 19, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Justin ParrenoRita A Kandel
Aug 26, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen E McGowan, Diann M McCoy
Mar 20, 2019·Cellular and Molecular Life Sciences : CMLS·Jakob VoelklIoana Alesutan
Mar 24, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Fei LiXianzhong Meng
Oct 22, 2020·PeerJ·Wanwan CaiWuzhou Yuan
Sep 4, 2017·Experimental Cell Research·Ece ÖztürkMarcy Zenobi-Wong
May 11, 2021·Frontiers in Cardiovascular Medicine·Wenhong JiangXiao Qin

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