Soy Isoflavone Glycitin (4'-Hydroxy-6-Methoxyisoflavone-7-D-Glucoside) Promotes Human Dermal Fibroblast Cell Proliferation and Migration via TGF-β Signaling

Phytotherapy Research : PTR
Young Mee KimMoonjae Cho

Abstract

Glycitin is a soy isoflavone that exhibits antioxidant, antiallergic, and anti-osteoporosis activities. We investigated the effects of glycitin on dermal fibroblast proliferation and migration. Treatment of primary dermal fibroblasts with glycitin increased cell proliferation and migration. In addition, treatment with 20 μM glycitin for 24 h induced the synthesis of collagen type I and type III at both the mRNA and protein levels. Fibronectin was also increased by 20% after treatment. Matrix metalloproteinase-1 collagenase was decreased in the media after 24-h incubation with glycitin, and the synthesis of transforming growth factor-beta (TGF-β) mRNA increased approximately twofold in cells following glycitin treatment. Phosphorylation of Smad2 and Smad3 increased after 1 h of glycitin treatment, and phosphorylation continued for 24 h. Furthermore, the phosphorylated form of AKT was increased in glycitin-treated cells after 3 h and remained higher for 24 h. Thus, glycitin treatment produces anti-aging effects including increased total collagen in the culture media, decreased elastase, and decreased β-galactosidase. Together, these results indicate that glycitin stimulates TGF-β secretion, and the subsequent autocrine actions of...Continue Reading

References

Aug 1, 1995·The Journal of Investigative Dermatology·H S TalwarJ J Voorhees
Nov 24, 1999·Journal of Agricultural and Food Chemistry·T T SongP A Murphy
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·M A Nugent, R V Iozzo
Apr 27, 2000·Nature Cell Biology·E ZamirB Geiger
Aug 5, 2000·Journal of Natural Products·P G Pietta
Dec 29, 2000·Phytochemistry·J B Harborne, C A Williams
Feb 22, 2001·Annual Review of Physiology·W V Cardoso
Aug 8, 2002·The Journal of Investigative Dermatology·Ulpu Saarialho-KereJouko Lohi
Nov 28, 2002·Pharmacology & Therapeutics·Bent H Havsteen
Jul 25, 2003·American Journal of Physiology. Cell Physiology·L E B StuhrR K Reed
Aug 5, 2003·Planta medica·Paul CosArnold J Vlietinck
Oct 15, 2003·Free Radical Biology & Medicine·Jacek ZielonkaGrzegorz Grynkiewicz
Feb 28, 2004·Expert Reviews in Molecular Medicine·Steven R BeanesKang Ting
Mar 17, 2004·Free Radical Biology & Medicine·Thomas Walle
Feb 1, 2005·Plant Foods for Human Nutrition·L H YaoS S Chen
May 20, 2005·American Journal of Obstetrics and Gynecology·Bertha ChenMary Lake Polan
May 4, 2006·European Journal of Clinical Nutrition·G R Lange SkovgaardM L Sigler
May 9, 2007·Journal of Nutritional Science and Vitaminology·Toru IzumiAsahi Matsuyama
Jul 7, 2009·Clinics·Alfeu Accorsi-NetoEdmund Baracat
Sep 22, 2010·Molecular Aspects of Medicine·Cesar G FragaPatricia I Oteiza
Oct 12, 2010·Aging·Joon-Il Jun, Lester F Lau
Aug 11, 2011·British Journal of Pharmacology·Francesca PolitoDomenica Altavilla
Jan 1, 2012·ISRN Molecular Biology·Jean-Jacques Lebrun

❮ Previous
Next ❯

Citations

Nov 25, 2016·Experimental and Therapeutic Medicine·Liyan ZhangDan Tian
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maobi ZhuTomohiko Iwano
May 14, 2021·Journal of Enzyme Inhibition and Medicinal Chemistry·Katarzyna JakimiukMichał Tomczyk
Jul 13, 2021·Critical Reviews in Food Science and Nutrition·Saied A AboushanabElena G Kovaleva

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.