SP-A enhances uptake of bacillus Calmette-Guérin by macrophages through a specific SP-A receptor

The American Journal of Physiology
L F WeikertV L Shepherd

Abstract

Surfactant-associated protein A (SP-A) is a C-type lectin that is involved in surfactant metabolism as well as host defense functions in the lung. We have recently identified a receptor on macrophages [specific 210-kDa SP-A receptor (SPR210)] that binds SP-A. In the current study we have investigated the role of SP-A in mediating uptake of bacillus Calmette-Guérin (BCG) by rat macrophages and human monocytes and have examined the role of the macrophage SPR210 in this process. 125I-labeled SP-A bound BCG in a Ca(2+)-, carbohydrate-, and dose-dependent manner. To examine association of SP-A-BCG complexes with macrophages, BCG were opsonized with SP-A and were incubated with rat bone marrow-derived macrophages (RBMM), rat alveolar macrophages (RAM), or human monocytes at a 1-to-1 ratio for 4 h. The cells were washed, fixed in formalin, and stained with auramine-rhodamine. Cell-associated organisms were enumerated by fluorescent microscopy. The percentage of cells with one or more associated BCG was increased by SP-A from 27% of RBMM with BCG alone to 54% with SP-A-BCG complexes; 1-16% in RAM; and 39-67% in human monocytes. This enhanced uptake was dependent on the dose of SP-A, with maximal increases seen with 10 micrograms/ml. El...Continue Reading

Citations

Feb 22, 2001·Annual Review of Physiology·E Crouch, J R Wright
Jan 27, 2004·Infection and Immunity·Jeffrey A GoldMichael D Weiden
Aug 5, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·L F WeikertV L Shepherd
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·J M Hickman-DavisJ R Wright
Dec 1, 2001·American Journal of Respiratory Cell and Molecular Biology·T R Korfhagen
Apr 5, 2002·Clinical Microbiology Reviews·Reinout van CrevelJos W M van der Meer
Jan 14, 2003·Annual Review of Immunology·Uffe HolmskovJens C Jensenius
Jul 16, 2005·American Journal of Respiratory and Critical Care Medicine·David S WilkesHerbert Y Reynolds
Jan 5, 2005·Nature Reviews. Immunology·Jo Rae Wright
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Isabelle VergneVojo Deretic
Nov 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·K G BrinkerJ R Wright
Nov 20, 2004·American Journal of Respiratory Cell and Molecular Biology·Sadis Matalon, Jo Rae Wright
Jan 28, 2021·Medicina·Heena Jagatia, Anthony G Tsolaki
Jul 15, 2004·Respiratory Medicine·Jianqiang DingDavid S Phelps

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