SP-A1 and SP-A2 variants differentially enhance association of Pseudomonas aeruginosa with rat alveolar macrophages

American Journal of Physiology. Lung Cellular and Molecular Physiology
Anatoly N MikerovJoanna Floros

Abstract

Chronic airway inflammation caused by Pseudomonas aeruginosa is an important feature of cystic fibrosis (CF). Surfactant protein A (SP-A) enhances phagocytosis of P. aeruginosa. Two genes, SP-A1 and SP-A2, encode human SP-A. We hypothesized that genetically determined differences in the activity of SP-A1 and SP-A2 gene products exist. To test this, we studied association of a nonmucoid P. aeruginosa strain (ATCC 39018) with rat alveolar macrophages in the presence or absence of insect cell-expressed human SP-A variants. We used two trios, each consisting of SP-A1, SP-A2, and their coexpressed SP-A1/SP-A2 variants. We tested the 6A(2) and 6A(4) alleles (for SP-A1), the 1A(0) and 1A alleles (for SP-A2), and their respective coexpressed SP-A1/SP-A2 gene products. After incubation of alveolar macrophages with P. aeruginosa in the presence of the SP-A variants at 37 degrees C for 1 h, the cell association of bacteria was assessed by light microscopy analysis. We found 1) depending on SP-A concentration and variant, SP-A2 variants significantly increased the cell association more than the SP-A1 variants (the phagocytic index for SP-A1 was approximately 52-95% of the SP-A2 activity); 2) coexpressed variants at certain concentrations w...Continue Reading

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Citations

May 24, 2007·Medical Microbiology and Immunology·Anatoly N MikerovJoanna Floros
Dec 7, 2010·Immunologic Research·Mashael Al-HegelanJohn W Hollingsworth
Jul 26, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ying WangMonica Kraft
Jun 16, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shirley R BruceJoseph L Alcorn
Feb 3, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Guirong WangJoanna Floros
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Patricia Silveyra, Joanna Floros
May 4, 2012·Swiss Medical Weekly·Patricia Silveyra, Joanna Floros
Apr 28, 2009·Critical Reviews in Eukaryotic Gene Expression·Joanna FlorosAnatoly N Mikerov
Jan 31, 2012·Microbial Pathogenesis·Anatoly N MikerovJoanna Floros
Jun 5, 2007·Immunobiology·Grith Lykke SorensenUffe Holmskov
Aug 11, 2016·Biophysical Journal·Elena Lopez-RodriguezJesus Perez-Gil
Jun 24, 2008·International Journal of Toxicology·Tsun-Cheng Kuo
Oct 27, 2005·American Journal of Physiology. Renal Physiology·Qiusheng TongJames D Stockand
Apr 8, 2006·American Journal of Physiology. Renal Physiology·Oleh PochynyukJames D Stockand
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Dec 26, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hephzibah Rani S TagaramJoanna Floros
Jul 1, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Claudette JeulinFrancelyne Marano
Jun 19, 2009·American Journal of Physiology. Renal Physiology·W B MelenhorstH van Goor
May 6, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Patricia SilveyraJoanna Floros
Jul 25, 2014·Experimental Lung Research·Patricia SilveyraJoanna Floros
Mar 9, 2007·American Journal of Physiology. Renal Physiology·Akiyuki TarunoYoshinori Marunaka
Jul 30, 2019·Frontiers in Immunology·George T NoutsiosJoanna Floros
Nov 6, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anatoly N MikerovJoanna Floros
Mar 20, 2009·American Journal of Physiology. Renal Physiology·Lian LiuDouglas C Eaton
Oct 5, 2007·American Journal of Physiology. Renal Physiology·Oleh PochynyukJames D Stockand
May 24, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Georgios T NoutsiosJoanna Floros
Oct 19, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nikolaos TsotakosJoanna Floros
Apr 1, 2015·Terapevticheskiĭ arkhiv·N A KovalkovaM I Voevoda
Sep 3, 2019·Frontiers in Immunology·Nithyananda ThorenoorJoanna Floros
Aug 16, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Patricia SilveyraJoanna Floros
Mar 26, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Georgios T NoutsiosJoanna Floros

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