SP1-induced upregulation of lncRNA PANDAR predicts adverse phenotypes in retinoblastoma and regulates cell growth and apoptosis in vitro and in vivo

Gene
Lijie ShengXiaoxue Sun

Abstract

Retinoblastoma (RB) is an intraocular malignancy for children and has a high mortality rate. Long non-coding RNAs (lncRNAs) are emerging as gene regulators and biomarkers in various malignancies. PANDAR is a novel cancer-related lncRNA that dysregulated in several types of cancers. However, its clinical value and potential effects on RB remains unclear. RT-qPCR was used to assess the relative expression of PANDAR in RB tissues and cells. Additionally, chromatin immunoprecipitation (ChIP) and luciferase reporter assays were performed to investigate whether SP1 could bind to the promoter region of PANDAR and activate its transcription. Furthermore, in vitro and in vivo studies were induced to elucidate the biological functions of PANDAR. The results indicated that PANDAR was increased in RB tissues and cells, and this upregulation was associated with advanced IIRC stage, positive optic nerve invasion, and lower differentiation grade in RB patients. In addition, SP1 could bind directly to the PANDAR promoter region and activate its transcription. Furthermore, PANDAR silencing yielded tumor suppressive effects both in vitro and in vivo. Importantly, PANDAR protects against apoptosis partly by affecting Bcl-2/caspase-3 pathway. Ulti...Continue Reading

Citations

Oct 3, 2019·International Journal of Cancer. Journal International Du Cancer·Jun-Jun QiuKe-Qin Hua
Oct 17, 2019·Frontiers in Genetics·Christopher M SmithGyorgy Hutvagner
Oct 29, 2019·Cancer Biotherapy & Radiopharmaceuticals·Lei ChenMengyi Huang
Dec 5, 2019·Frontiers in Genetics·Meropi Plousiou, Ivan Vannini
Dec 8, 2020·Frontiers in Genetics·Xuedong ChenHongyan Ge
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Defeng QiFenghua Yu
Apr 5, 2021·Experimental Eye Research·Jinlu Liu, Xiaohan Qu
Feb 24, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xue-Zhi WuLei Feng
Apr 12, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhou SuJian Pei
Jun 17, 2019·Pathology, Research and Practice·Ming Yang, Wenbin Wei

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