SP1 regulates a human SNAP-25 gene expression

Journal of Neurochemistry
Fang CaiWeihong Song

Abstract

The synaptosomal-associated protein of 25 kDa (SNAP-25) is a pre-synaptic plasma membrane protein. SNAP-25 plays an important role in synaptic vesicle membrane docking and fusion, which is involved in the regulation of neurotransmitter release. SNAP-25 has been implicated in the pathogenesis of neuropsychiatric disorders including Schizophrenia, attention-deficit hyperactivity disorder and Alzheimer's disease. We cloned a 1584 bp segment of the 5' flanking region of the human SNAP-25 gene. A series of nested deletions of the 5' flanking region fragment were subcloned into the pGL3-basic luciferase reporter plasmid. N2A cells were transfected with the SNAP-25 promoter constructs and luciferase activity was measured as an indication of promoter activity. We identified a 188 bp fragment containing the transcription initiation site as the minimal region necessary for promoter activity. Several putative cis-acting elements including SP1, hypoxia inducible factor (HIF), cAMP-response element binding protein, T-cell factor/lymphocyte enhancer factor 1 (TCF/LEF1), AP1 and the signal transducer and activator of transcription-6 (STAT6) are found in the 5' flanking region of SNAP-25 gene. Transcriptional activation and gel shift assays sh...Continue Reading

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Citations

Mar 9, 2012·Journal of Molecular Neuroscience : MN·Ruitao WangWeihong Song
Nov 30, 2013·Journal of Neurochemistry·Ke WangWeihong Song
Mar 24, 2016·Molecular Brain·Juelu Wang, Weihong Song
Feb 19, 2011·The International Journal of Neuropsychopharmacology·Chia-Hsiung ChenWeihong Song
Jun 3, 2016·Neuron·Zhe WangCarles Vilariño-Güell
Nov 3, 2016·The International Journal of Neuroscience·Aneeqa Noor, Saadia Zahid
Jun 28, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Si ZhangWeihong Song
Jul 22, 2017·Journal of Alzheimer's Disease : JAD·Lindsey I Sinclair, Seth Love

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