Sp8 and Sp9, two closely related buttonhead-like transcription factors, regulate Fgf8 expression and limb outgrowth in vertebrate embryos

Development
Yasuhiko KawakamiJuan Carlos Izpisúa Belmonte

Abstract

Initiation and maintenance of signaling centers is a key issue during embryonic development. The apical ectodermal ridge, a specialized epithelial structure and source of Fgf8, is a pivotal signaling center for limb outgrowth. We show that two closely related buttonhead-like zinc-finger transcription factors, Sp8 and Sp9, are expressed in the AER, and regulate Fgf8 expression and limb outgrowth. Embryological and genetic analyses have revealed that Sp8 and Sp9 are ectodermal targets of Fgf10 signaling from the mesenchyme. We also found that Wnt/beta-catenin signaling positively regulates Sp8, but not Sp9. Overexpression functional analyses in chick unveiled their role as positive regulators of Fgf8 expression. Moreover, a dominant-negative approach in chick and knockdown analysis with morpholinos in zebrafish revealed their requirement for Fgf8 expression and limb outgrowth, and further indicate that they have a coordinated action on Fgf8 expression. Our study demonstrates that Sp8 and Sp9, via Fgf8, are involved in mediating the actions of Fgf10 and Wnt/beta-catenin signaling during vertebrate limb outgrowth.

References

Mar 7, 1996·Nature·P H CrossleyG R Martin
Jun 21, 1996·The Journal of Biological Chemistry·D M OrnitzM Goldfarb
Mar 7, 1998·Developmental Biology·J CapdevilaR L Johnson
Jun 17, 1998·Genes & Development·G R Martin
Jan 23, 1999·Nature Genetics·K SekineS Kato
Jun 1, 1999·Mechanisms of Development·H Grandel, S Schulte-Merker
Aug 24, 1999·Nucleic Acids Research·S Philipsen, G Suske
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·E ArmanP Lonai
Oct 12, 2000·Nature·K I PinsonW C Skarnes
Dec 2, 2000·Nature Genetics·A M Moon, M R Capecchi
Dec 2, 2000·Nature Genetics·M LewandoskiG R Martin
Jul 13, 2001·Current Opinion in Genetics & Development·C Tickle, A Münsterberg
Nov 1, 2001·Annual Review of Cell and Developmental Biology·J Capdevila, J C Izpisúa Belmonte
Nov 13, 2001·Developmental Cell·M MukhopadhyayH Westphal
Feb 28, 2002·Molecular Biology and Evolution·Kevin J Kolell, Douglas L Crawford
Mar 29, 2002·Journal of Morphology·Cheryll Tickle
Jun 11, 2002·Journal of Molecular Biology·Vincent BrondaniFrançois Hamy
Jun 18, 2002·Nature·Dae-gwon AhnRobert K Ho
Oct 2, 2002·Molecular and Cellular Endocrinology·Peter Bouwman, Sjaak Philipsen
Oct 3, 2002·American Journal of Medical Genetics·Cheryll Tickle
Feb 1, 2003·Nature Reviews. Genetics·Lee Niswander
May 27, 2003·Nature Cell Biology·Yasuhiko KawakamiJuan Carlos Izpisúa Belmonte
Jun 24, 2003·Genome Research·Timothy RavasiUNKNOWN GSL Members
Oct 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sheila M BellWilliam J Scott
Nov 5, 2003·Genes & Development·Dieter TreichelAhmed Mansouri
Jan 28, 2004·Birth Defects Research. Part C, Embryo Today : Reviews·Yingzi Yang

❮ Previous
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Citations

Apr 12, 2005·Genomics·Guntram SuskeSjaak Philipsen
Feb 14, 2006·Nature Chemical Biology·Hendrik LueschPeter G Schultz
Dec 5, 2009·The Journal of Biological Chemistry·Jianzhen XieJonathan M Horowitz
Jun 1, 2005·Development, Growth & Differentiation·Chengtian Zhao, Anming Meng
Jun 26, 2007·Development, Growth & Differentiation·Nadia Mercader
Jul 31, 2007·Development, Growth & Differentiation·Jesús C Casanova, Juan José Sanz-Ezquerro
May 4, 2010·BMC Genomics·Arun SeetharamGary W Stuart
Sep 23, 2011·Development·Yasuhiko KawakamiJuan Carlos Izpisua Belmonte
Aug 20, 2011·PLoS Genetics·Sylvain W Lapan, Peter W Reddien
Apr 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hyeyoung A ChungRichard M Harland
Jul 1, 2011·Journal of Biomedicine & Biotechnology·Luisa GuerriniGiorgio R Merlo
Feb 24, 2009·Biochemical and Biophysical Research Communications·Wakako YamadaKeiyo Takubo
Dec 19, 2006·Current Opinion in Chemical Biology·Nidhi M Gangadhar, Brent R Stockwell
Jan 9, 2008·Birth Defects Research. Part A, Clinical and Molecular Teratology·Diana M Juriloff, Muriel J Harris
Mar 3, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christopher T GordonPeter G Farlie
May 7, 2008·The Journal of Comparative Neurology·Jeremy A Cholfin, John L R Rubenstein
Nov 22, 2008·Evolution & Development·Sayuri Yonei-TamuraKoji Tamura
Mar 9, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Junji ItouYasuhiko Kawakami
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shimei ZhuFrancesca Gori
Nov 23, 2011·The European Journal of Neuroscience·Paul G Anastasiades, Simon J B Butt
Mar 13, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Naoki NomuraKoji Tamura
Nov 27, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Marcela BuchtováJoy M Richman
Feb 14, 2006·Biochemical and Biophysical Research Communications·Chengtian ZhaoAnming Meng
Dec 29, 2005·Biochemical and Biophysical Research Communications·Yongxin ChenHiroyuki Aburatani
Jun 28, 2005·Current Opinion in Genetics & Development·James W Mahaffey
Mar 17, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·David M Ornitz, Nobuyuki Itoh
Nov 21, 2012·Developmental Biology·Thomas IngGerold Schubiger
Feb 14, 2012·Neuroscience Letters·Tadasuke Tsukiyama, Terry P Yamaguchi
Jul 23, 2013·Developmental Biology·Abigail D KasbergS Steven Potter
Nov 17, 2009·Developmental Biology·Ana TalamilloMaria A Ros
May 18, 2011·Developmental Biology·Ana Raquel TomásJoaquín Rodríguez-León

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