Space-clamp problems when voltage clamping neurons expressing voltage-gated conductances

Journal of Neurophysiology
Dan Bar-Yehuda, Alon Korngreen

Abstract

The voltage-clamp technique is applicable only to spherical cells. In nonspherical cells, such as neurons, the membrane potential is not clamped distal to the voltage-clamp electrode. This means that the current recorded by the voltage-clamp electrode is the sum of the local current and of axial currents from locations experiencing different membrane potentials. Furthermore, voltage-gated currents recorded from a nonspherical cell are, by definition, severely distorted due to the lack of space clamp. Justifications for voltage clamping in nonspherical cells are, first, that the lack of space clamp is not severe in neurons with short dendrites. Second, passive cable theory may be invoked to justify application of voltage clamp to branching neurons, suggesting that the potential decay is sufficiently shallow to allow spatial clamping of the neuron. Here, using numerical simulations, we show that the distortions of voltage-gated K(+) and Ca(2+) currents are substantial even in neurons with short dendrites. The simulations also demonstrate that passive cable theory cannot be used to justify voltage clamping of neurons due to significant shunting to the reversal potential of the voltage-gated conductance during channel activation. S...Continue Reading

References

Oct 1, 1992·Physiological Reviews·W RallI Segev
Jan 1, 1992·Methods in Enzymology·C M Armstrong, W F Gilly
Sep 1, 1989·Pflügers Archiv : European journal of physiology·F A EdwardsT Takahashi
Oct 1, 1985·The Journal of Physiology·G J AugustineS J Smith
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jun 1, 1993·Pflügers Archiv : European journal of physiology·G J StuartB Sakmann
Jan 1, 1996·Annual Review of Neuroscience·D JohnstonB R Cristie
Aug 15, 1997·Neural Computation·M L Hines, N T Carnevale
Jul 25, 2000·Neural Computation·M L Hines, N T Carnevale
May 4, 2002·Nature Reviews. Neuroscience·Michele Migliore, Gordon M Shepherd
Mar 4, 2003·Journal of Neurophysiology·Andreas T SchaeferArnd Roth
May 27, 2003·Journal of Computational Neuroscience·Daniel K Hartline, Ann M Castelfranco
May 29, 2003·Biophysical Journal·Andreas T SchaeferAlon Korngreen
Mar 23, 2006·Nature Reviews. Neuroscience·Giorgio A Ascoli
Dec 13, 2006·The Journal of Physiology·Andreas T SchaeferAlon Korngreen

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Citations

May 7, 2008·Pflügers Archiv : European journal of physiology·Bingxian WangJan D Huizinga
Aug 16, 2008·Pflügers Archiv : European journal of physiology·M MilleK Hilber
Mar 10, 2012·Journal of Molecular Histology·Stefan WeigelAndreas Offenhäusser
Jan 19, 2011·Journal of Computational Neuroscience·Eric B HendricksonDieter Jaeger
Jun 26, 2008·Nature Neuroscience·Nelson Spruston, Daniel Johnston
Sep 24, 2010·American Journal of Physiology. Cell Physiology·Mingjie TongR Keith Duncan
Dec 12, 2012·Journal of Neurophysiology·Jokubas ŽiburkusSteven J Schiff
Apr 2, 2011·PLoS Computational Biology·Tamar PashutAlon Korngreen
Jun 25, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aleksander W BalloDirk Bucher
Nov 16, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ping LvEbenezer N Yamoah
Jun 4, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lorin S MilescuJeffrey C Smith
Apr 12, 2016·Communicative & Integrative Biology·Hojeong Kim, C J Heckman
May 19, 2009·European Journal of Pharmacology·Ervice PouokamMartin Diener
Jul 15, 2009·The Journal of Physiology·Desdemona FrickerRichard Miles
Dec 3, 2008·The European Journal of Neuroscience·Dan Bar-YehudaAlon Korngreen
Mar 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Rishi K AlluriJeremy Wilkerson
Jul 11, 2012·The European Journal of Neuroscience·Crawford I P Winlove, Alan Roberts
Apr 14, 2016·Neurobiology of Disease·Kashif MahfoozJohn F Wesseling
Jun 3, 2014·Psychoneuroendocrinology·Peter KoppensteinerMasayuki Sekiguchi
May 22, 2016·Journal of Neuroscience Methods·Josh MerelLiam Paninski
Nov 5, 2016·Neurotoxicology·Leonardo Lara-ValderrábanoEmilio J Galván
Apr 14, 2017·The Journal of Physical Chemistry Letters·Hyeon Jeong LeeJi-Xin Cheng
Oct 19, 2017·Cerebral Cortex·Mieko MorishimaYasuo Kawaguchi
Apr 19, 2018·ELife·Richard Burkett Dewell, Fabrizio Gabbiani
Mar 31, 2017·Journal of Neurophysiology·Min-Yu SunSteven J Mennerick
Apr 29, 2018·Hippocampus·Gabriel Herrera-López, Emilio J Galván
Feb 2, 2019·The European Journal of Neuroscience·Victor S C WongDianna E Willis
Feb 13, 2019·The Journal of Physiology·Melina P da SilvaBenedito H Machado
Jun 2, 2012·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Steven J Zottoli
Oct 20, 2017·Journal of Neurophysiology·Briana J CarrollRichard L Hyson

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