Spaciotemporal association and bone morphogenetic protein regulation of sclerostin and osterix expression during embryonic osteogenesis

Endocrinology
Yoshio OhyamaMasaki Noda

Abstract

Sclerostin (SOST), a member of the cystine-knot superfamily, is essential for proper skeletogenesis because a loss-of-function mutation in the SOST gene results in sclerosteosis featured with massive bone growth in humans. To understand the function of SOST in developmental skeletal tissue formation, we examined SOST gene expression in embryonic osteogenesis in vitro and in vivo. During osteoblastic differentiation in primary calvarial cells, the levels of SOST expression were increased along with those of alkaline phosphatase activity and nodule formation. In situ hybridization study revealed that SOST mRNA expression was observed in the digits in embryonic 13-d limb buds, and SOST expression was observed in osteogenic front in embryonic 16.5-d postcoitus embryonic calvariae, and this expression persisted in the peripheral area of cranial bone in the later developmental stage (embryonic 18.5-d post coitum). These temporal and spacial expression patterns in vivo and in vitro were in parallel to those of osterix (Osx), which is a critical transcriptional factor for bone formation. Similar coexpression of SOST and Osx mRNA was observed when the primary osteoblastic calvarial cells were cultured in the presence of bone morphogenet...Continue Reading

References

Feb 1, 1984·Clinical Genetics·P BeightonA van der Wouden
Mar 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A NifujiM Noda
Oct 27, 1997·Science·E LauferL Niswander
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·W ShawlotR R Behringer
Apr 20, 1999·Developmental Biology·J J PearceJ Rossant
Jul 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J E Aubin
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·J MarcelinoM L Warman
Dec 4, 2001·Mechanisms of Development·J OgitaH Koseki
Oct 12, 2002·Developmental Biology·Wendy Balemans, Wim Van Hul
Feb 20, 2003·Nature Biotechnology·Jason W MyersJames E Ferrell
Mar 21, 2003·Journal of Cellular Biochemistry·K YagiMasaki Noda

❮ Previous
Next ❯

Citations

May 5, 2005·Cytokine & Growth Factor Reviews·Rutger L van BezooijenClemens W G M Löwik
Apr 1, 2009·Therapeutic Advances in Musculoskeletal Disease·Dayong Guo, Lynda F Bonewald
Aug 2, 2013·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·B García-FontanaM Muñoz-Torres
May 18, 2010·Calcified Tissue International·M J C MoesterR L van Bezooijen
Jul 18, 2014·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·R Sapir-Koren, G Livshits
May 23, 2012·Medicina clínica·Guillermo AlonsoManuel Muñoz-Torres
Jun 16, 2009·Molecular and Cellular Endocrinology·Frances Milat, Kong Wah Ng
Apr 25, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kaoru Washio-OikawaMasaki Noda
May 12, 2006·Periodontology 2000·Francis J HughesGianluca Martuscelli
Feb 12, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Gerald J AtkinsDavid M Findlay
Feb 26, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jin ZhangJake Chen
Oct 31, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nobuhiro KamiyaYuji Mishina
Apr 1, 2006·Gene·Yasuhiko NishioHicham Drissi
Jul 6, 2012·Expert Opinion on Therapeutic Targets·Maria GkotzamanidouEvangelos Terpos
Oct 7, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yue ZhangGordana Vunjak-Novakovic
Jun 4, 2014·Therapeutic Advances in Musculoskeletal Disease·Gaël Y Rochefort
Nov 10, 2013·The Journal of Clinical Endocrinology and Metabolism·Roland KocijanHeinrich Resch
Feb 11, 2005·The Journal of Biological Chemistry·Christopher J LengnerGary S Stein
Oct 25, 2016·Bone·Aimy Sebastian, Gabriela G Loots
Apr 21, 2021·Journal of Oral Biosciences·Nisha AshifaSivapragasam Srinivasan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.