SPAG11/isoform HE2C, an atypical anionic beta-defensin-like peptide

Peptides
Hans Henning von HorstenC Kirchhoff

Abstract

A human caput epididymidal cDNA, HE2C, was cloned based on its homology to the known chimpanzee counterpart, suggesting that the encoded beta-defensin-like peptide represented a conserved component of the innate epididymidal epithelial defense system in primates. An approximately 6kDa HE2- related peptide was co-purified together with other HE2 isoforms from human seminal plasma by affinity chromatography. By its antibody reactivity as shown by Western blot analysis, this peptide was distinct from the more abundant HE2 isoforms and was concluded to correspond to HE2C. Similar to other HE2-encoded isoforms, the endogenous HE2C was proteolytically processed from a larger precursor by a furin-like prohormone convertase. This was confirmed by N-terminal sequencing. In order to study the structural and functional properties of HE2C it was recombinantly expressed in insect cells. Post-translational processing also occurred within these cells, yielding the mature processed HE2C peptide. Correct disulfide bonding of the recHE2C peptide was shown by p-aminophenylarsineoxide(PAPAO)-agarose binding assay. Purified recHE2C strongly bound to Escherichia coli DH5alpha and Bacillus subtilis; however, it did not exhibit microbicidal activity w...Continue Reading

References

Mar 21, 1991·Journal of Immunological Methods·R I LehrerP Eisenhauer
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
May 1, 1994·Journal of Reproductive Immunology·P Pöllänen, T G Cooper
Jan 1, 1993·Annual Review of Immunology·R I LehrerT Ganz
Feb 7, 1998·The Journal of Biological Chemistry·S IchikawaF Inagaki
Jan 25, 2002·Nature·Michael Zasloff
Aug 24, 2002·Biology of Reproduction·Hans Henning von HorstenChristiane Kirchhoff
Apr 3, 2003·Microscopy Research and Technique·Jean-Louis DacheuxFrançoise Dacheux

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Citations

Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
Jun 19, 2009·Biology of Reproduction·Yashwanth RadhakrishnanFrank S French
Aug 12, 2009·BMC Genomics·Benoît GuyonnetJean-Luc Gatti
Apr 29, 2006·Reproductive Biology and Endocrinology : RB&E·Suresh YenuguSusan H Hall
Jan 13, 2015·Systems Biology in Reproductive Medicine·Ganapathy Narmadha, Suresh Yenugu
Nov 27, 2008·American Journal of Reproductive Immunology : AJRI·Michael A PalladinoDaniel Plaska
Sep 26, 2006·Molecular and Cellular Endocrinology·D ShayuA J Rao
Oct 6, 2005·Journal of Biochemistry and Molecular Biology·Yoonkyung Park, Kyung-Soo Hahm
Mar 21, 2015·Amino Acids·Lin WeiHailong Yang
Sep 14, 2006·Molecular Diversity·Colin A SempleJulia R Dorin

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